Results 81 to 90 of about 166,210 (263)

Programmable Nanobody‐Targeting Chimeras Enable Intracellular Viral Protein Degradation

Advanced Science, EarlyView.
Nab‐TAC enables targeted degradation of HBV surface antigen (HBsAg) in vivo. By fusing nanobody‐based recognition domains with programmable proteasome‐recruiting degradation signals, Nab‐TAC reduces HBsAg levels in a hydrodynamic HBV mouse model. ABSTRACT Chronic hepatitis B virus (HBV) infection remains a major global health challenge, largely because
Max Yu‐Chen Pan, Ting‐Hui Lee, Tzu‐Ning Peng, Eva Yi‐Hsuan Wu, Yu‐Ting Chiu, Chih‐Chien Chiu, Hong Vinh Nguyen, Yuan‐Shao Pao, Ming‐Hong Chao, Richard Kuan‐Lin Lee, Han‐Chung Wu, Lily Hui‐Ching Wang   +11 more
wiley   +1 more source

The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein

mBio, 2018
Zika virus (ZIKV) infection of neural progenitor cells (NPCs) in utero is associated with neurological disorders, such as microcephaly, but a detailed molecular understanding of ZIKV-induced pathogenesis is lacking.
Krystal A. Fontaine, Kristoffer E. Leon, Mir M. Khalid, Sakshi Tomar, David Jimenez-Morales, Mariah Dunlap, Julia A. Kaye, Priya S. Shah, Steve Finkbeiner, Nevan J. Krogan, Melanie Ott   +10 more
doaj   +1 more source

Molecular Studies on Bromovirus Capsid Protein

Virology, 1997
Specific interactions are likely to occur between the highly conserved N-proximal arginine-rich motif (ARM) of Brome mosaic virus (BMV) coat protein (CP) and each of three genomic RNAs and a single subgenomic RNA during in vivo encapsidation. To characterize these interactions, three independent deletions were engineered into a biologically active ...
Osman, Fatima, Grantham, George L, Rao, A.L.N   +2 more
openaire   +2 more sources

Untangling Inositol (Pyro)Phosphate Biology Through Emerging Technologies

Advanced Science, EarlyView.
Inositol (pyro)phosphates represent a vital class of intracellular messengers that govern multiple biological processes. The last decade has experienced a surge of interest in the functions of this class of signaling molecules, primarily due to the introduction of several innovative analytical technologies.
Adolfo Saiardi, Mengsi Lu, Yue Zhang, Anuj Shukla, An‐Li Andrea Ko, Henning J. Jessen   +5 more
wiley   +1 more source

AAV capsid sites breakdown: large protein insertions impact on vector dynamics

Frontiers in Bioengineering and Biotechnology
IntroductionAdeno-associated virus (AAV) vectors are one of the most used gene delivery systems, and several capsid engineering strategies have been followed to further improve their performance.
Mariana V. Ferreira, Mariana V. Ferreira, Marina Curto, Marina Curto, André Nascimento, André Nascimento, Micael C. Freitas, Pedro M. F. Sousa, Filipe Freire, Tiago M. Bandeiras, Cristina Peixoto, Cristina Peixoto, Ana Sofia Coroadinha, Ana Sofia Coroadinha   +13 more
doaj   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Overcoming Transmission Barriers: A Dual‐Functional Adhesive Hydrogel for Direct Monkeypox Virus Neutralization and Wound Healing

Advanced Science, EarlyView.
MPXV infection leads to persistent viral replication and severe local inflammation, while existing treatments cannot simultaneously remove viruses and repair injured tissue. This study constructs Polymer@mPDA@Gel hydrogel via a two‐step hierarchical assembly method: mPDA anchors photothermal polymers to form antiviral photothermal subunits, which are ...
Ming Zhang, Jiaxin Tian, Xiaolong Zhu, Xiao Li, Xiangshu Qiu, Yuanyuan Li, Ben Zhong Tang, Ning Shi   +7 more
wiley   +1 more source

Capsid and Procapsid Proteins of a Bovine Enterovirus

Journal of General Virology, 1971
Summary A bovine enterovirus (serotype VG-5-27) was grown in BHK 21 cells and purified using gel filtration and sedimentation procedures. Infective particles sedimented at 165s and the empty capsids or procapsids at 75s. Proteins extracted from each type of component were separated by polyacrylamide-gel electrophoresis.
M D, Johnston, S J, Martin
openaire   +2 more sources

Computer Vision Pipeline for Image Analysis for Freeze‐Fracture Electron Microscopy: Rosette Cellulose Synthase Complexes Case

Advanced Intelligent Discovery, EarlyView.
This paper presents a computer vision (deep learning) pipeline integrating YOLOv8 and YOLOv9 for automated detection, segmentation, and analysis of rosette cellulose synthase complexes in freeze‐fracture electron microscopy images. The study explores curated dataset expansion for model improvement and highlights pipeline accuracy, speed ...
Siri Mudunuri, Leala Carbonneau, Eric M. Roberts, Alison W. Roberts, Candace H. Haigler, Alexey V. Gulyuk, Yaroslava G. Yingling   +6 more
wiley   +1 more source

Random Insertion of mCherry Into VP3 Domain of Adeno-associated Virus Yields Fluorescent Capsids With no Loss of Infectivity

Molecular Therapy: Nucleic Acids, 2012
Adeno-associated virus (AAV)-derived vectors are promising gene delivery systems, and a number of design strategies have been pursued to improve their performance.
Justin Judd, Fang Wei, Peter Q Nguyen, Lawrence J Tartaglia, Mavis Agbandje-McKenna, Jonathan J Silberg, Junghae Suh   +6 more
doaj   +1 more source