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Carbohydrate-active enzymes (CAZymes) in the gut microbiome
Nature Reviews Microbiology, 2022The 1013-1014 microorganisms present in the human gut (collectively known as the human gut microbiota) dedicate substantial percentages of their genomes to the degradation and uptake of carbohydrates, indicating the importance of this class of molecules.
Jacob F Wardman +2 more
exaly +3 more sources
Building mutational bridges between carbohydrate-active enzymes
Current Opinion in Biotechnology, 2022 The commercial value of specialty carbohydrates and glycosylated compounds has sparked considerable interest in the synthetic potential of carbohydrate-active enzymes (CAZymes). Protein engineering methods have proven to be highly successful in expanding the range of glycosylation reactions that these enzymes can perform efficiently and cost ...
Jorick, Franceus +2 more
openaire +3 more sources
Journal of the Science of Food and Agriculture, 2006 Enzymes that degrade, modify, or create glycosidic bonds are involved in carbohydrate biosynthesis and remodelling. Microbial carbohydrate-active enzymes form the basis of current green technology in the food, feed, starch, paper and pulp industries and ...
Nathalie Juge, Birte Svensson
exaly +2 more sources
Role of carbohydrate-active enzymes in mycorrhizal symbioses
Essays in Biochemistry, 2023Abstract Mycorrhizal fungi form mutually beneficial interactions with a wide range of terrestrial plants. During this symbiosis, the associated fungus provides mineral nutrients, such as phosphorus and nitrogen, to its host plant in exchange of photosynthesis-derived carbohydrates.
Gong, Yuhua +3 more
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Enzyme promiscuity of carbohydrate active enzymes and their applications in biocatalysis
Current Opinion in Structural Biology, 2020The application of biocatalysis for the synthesis of glycans and glycoconjugates is a well-established and successful strategy, both for small and large scale synthesis. Compared to chemical synthesis, is has the advantage of high selectivity, but biocatalysis had been largely limited to natural glycans both in terms of reactivity and substrates.
Edward, Pallister +2 more
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A New Versatile Microarray-based Method for High Throughput Screening of Carbohydrate-active Enzymes
Journal of Biological Chemistry, 2015 Carbohydrate-active enzymes have multiple biological roles and industrial applications. Advances in genome and transcriptome sequencing together with associated bioinformatics tools have identified vast numbers of putative carbohydrate-degrading and ...
Silvia Vidal-Melgosa +2 more
exaly +2 more sources
Polymeric Iminosugars Improve the Activity of Carbohydrate-Processing Enzymes
Bioconjugate Chemistry, 2015Multivalent iminosugars have recently emerged as powerful tools to inhibit the activities of specific glycosidases. In this work, biocompatible dextrans were coated with iminosugars to form linear and ramified polymers with unprecedently high valencies (from 20 to 900) to probe the evolution of the multivalent inhibition as a function of ligand valency.
Brissonnet, Yoan +10 more
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Interaction of carbohydrates with alcohol dehydrogenase: Effect on enzyme activity
Journal of Bioscience and Bioengineering, 2015Alcohol dehydrogenase was covalently conjugated with three different oxidized carbohydrates i.e., glucose, starch and pectin. All the carbohydrates inhibited the enzyme. The inhibition was studied with respect to the inhibition rate constant, involvement of thiol groups in the binding, and structural changes in the enzyme. The enzyme activity decreased
Jadhav, Swati B. +6 more
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A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana
Plant Molecular Biology, 2001The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdown (glycoside hydrolases and ...
B, Henrissat, P M, Coutinho, G J, Davies
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