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Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes
2021Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyze their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their underpinning physiological roles. Here we survey recent accomplishments for accessing defined carbohydrate structures, suitably equipped with FRET ...
Singh, Meenakshi +3 more
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Electrostatic studies of carbohydrate active enzymes
1995Abstract Most charged or titratable residues reside on the protein surface. Each of such residues will exhibit a titration behaviour that is dependent on the local context around the residue. As a consequence both pH, salt and docking of a ligand into the binding cavity of a protein can be expected to alter the protein electrostatics.
Antonio Baptista +5 more
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Exploring the sheep rumen microbiome for carbohydrate-active enzymes
Antonie van Leeuwenhoek, 2015The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal's digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this
Lucas Dantas, Lopes +8 more
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Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes
Applied Biochemistry and Biotechnology, 2010Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009.
Phillip, Brumm +7 more
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Interaction of carbohydrates with alcohol dehydrogenase: Effect on enzyme activity
Journal of Bioscience and Bioengineering, 2015Alcohol dehydrogenase was covalently conjugated with three different oxidized carbohydrates i.e., glucose, starch and pectin. All the carbohydrates inhibited the enzyme. The inhibition was studied with respect to the inhibition rate constant, involvement of thiol groups in the binding, and structural changes in the enzyme. The enzyme activity decreased
Jadhav, Swati B. +6 more
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Cazypedia : carbohydrate-active enzymes, an introduction
2011CAZypedia is an online, community-driven effort to assemble a comprehensive encyclopedia of the enzymes and binding proteins involved in the synthesis and degradation of complex carbohydrates. An evolving encyclopedia of carbohydrate-active enzymes – ”CAZymes” CAZypedia is inspired by, and closely connected with, the actively curated CAZy Database (www.
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Carbohydrate-active enzyme annotation in microbiomes using dbCAN
AbstractCAZymes or carbohydrate-active enzymes are critically important for human gut health, lignocellulose degradation, global carbon recycling, soil health, and plant disease. We developed dbCAN as a web server in 2012 and actively maintain it for automated CAZyme annotation.Jinfang, Zheng +6 more
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Carbohydrates and Carbohydrate-Active enZymes (CAZyme): An overview
2023Parmeshwar Vitthal Gavande +2 more
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Computational methods in the study of carbohydrates and carbohydrate-active enzymes
2018Different programs and methods were employed to superimpose protein structures, using members of four very different protein families as test subjects, and the results of these efforts were compared. Algorithms based on human identification of key amino acid residues on which to base the superpositions were nearly always more successful than programs ...
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