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Mass spectrometry for structural elucidation and sequencing of carbohydrates
TrAC Trends in Analytical Chemistry, 2021Abstract Carbohydrates are the most abundant organic compounds in nature, widely distributed in animals, plants and microorganisms. In order to understanding their structure properties, the following critical aspects should be revealed: a) building blocks (compositional monosaccharide); b) glycosidic linkages and anomeric configuration; c) sequence ...
Shao-Ping Li +5 more
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GlycoCT—a unifying sequence format for carbohydrates
Carbohydrate Research, 2008As part of the EUROCarbDB project (www.eurocarbdb.org) we have carefully analyzed the encoding capabilities of all existing carbohydrate sequence formats and the content of publically available structure databases. We have found that none of the existing structural encoding schemata are capable of coping with the full complexity to be expected for ...
Stephan Herget +3 more
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Journal of Chromatography A, 1996
Carbohydrate analysis has traditionally been viewed as a specialty science, performed only in a few well-established laboratories using conventional carbohydrate analysis technology (e.g. NMR, gas chromatography-mass spectroscopy, high-performance liquid chromatography, capillary electrophoresis) combined with the specialized technical training that ...
Christopher M. Starr +4 more
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Carbohydrate analysis has traditionally been viewed as a specialty science, performed only in a few well-established laboratories using conventional carbohydrate analysis technology (e.g. NMR, gas chromatography-mass spectroscopy, high-performance liquid chromatography, capillary electrophoresis) combined with the specialized technical training that ...
Christopher M. Starr +4 more
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Carbohydrate storage in anaerobic sequencing batch reactors
Water Research, 2007This study demonstrates the accumulation and degradation of trehalose as a storage compound in a glucose-fed anaerobic sequencing batch reactor (ASBR). One hour after substrate addition, only 40% of the added organic matter (as chemical oxygen demand, COD) was accounted for by the cumulative methane production and soluble COD remaining in the reactor ...
Eberhard Morgenroth +3 more
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Primary sequence analysis and representation techniques in carbohydrates
Bioinformatics, 1993Sequence similarity calculations of carbohydrates present several problems which must be addressed if a computer implementation is to be achieved. These problems range from the computational representation of the complex carbohydrate structure to the method by which the comparison of residue and linkage is to be made. This paper therefore discusses the
Robert A. Rastall +4 more
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Nature Methods, 2017
A technique combining mass spectrometry and infrared spectroscopy shows promise for unambiguous sequencing of linear oligosaccharides.
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A technique combining mass spectrometry and infrared spectroscopy shows promise for unambiguous sequencing of linear oligosaccharides.
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Carbohydrate-Lectin Recognition of Sequence-Defined Heteromultivalent Glycooligomers
Journal of the American Chemical Society, 2014Multivalency as a key principle in nature has been successfully adopted for the design and synthesis of artificial glycoligands by attaching multiple copies of monosaccharides to a synthetic scaffold. Besides their potential in various applied areas, e.g.
Stephan Schmidt +10 more
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Hybrid method sequences carbohydrates
C&EN Global Enterprise, 2017Methods for routine sequencing of carbohydrates lag behind those for sequencing nucleic acids and proteins. The lack of such methods makes fully characterizing carbohydrates difficult. Sequencing a carbohydrate is a challenge because many of the sugar building blocks are isomers of one another, making it hard to distinguish between them, and the ...
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