Results 31 to 40 of about 356 (148)
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Carnets de Géologie, 2006 The fossil renamed here was first described in 1959 as Aeolisaccus kotori Radoičić, a new species of a problematic fossil worm, Aeolisaccus Elliott. In 1975 De Castro recognized the true relationships of this microbial fossil: a cyanobacterium related ...
Golubic Stjepko +2 more
doaj
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Quaternary glacial cycles: Karst processes and the global CO2 budget
Acta Carsologica, 2013 Extensive research has been conducted investigating the relationship between karst processes, carbonate deposition and the global carbon cycle. However, little work has been done looking into the relationship between glaciations, subsequent sea level ...
Erik B. Larson, John E. Mylroie
doaj +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo
FEBS Open Bio, EarlyView.To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley +1 more source
Rivista Italiana di Paleontologia e Stratigrafia, 2017 The Ladinian rocks of Central Lombardy consist of carbonate platforms (Esino Formation) subdivided by intraplatform troughs represented either by dark, well bedded limestones, marls and dolomites of poorly oxic to anoxic environment (Perledo-Varenna and ...
MAURIZIO GAETANI +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada +7 more
wiley +1 more source
TERRESTRIAL ECOSYSTEMS ON THE MESOZOIC PERI-ADRIATIC CARBONATE PLATFORMS: THE VERTEBRATE EVIDENCE
Publicación Electrónica de la Asociación Paleontológica Argentina, 2015 The remains of Mesozoic carbonate platforms outcrop along the margins of the Adriatic Sea and the mountain chains surrounding them in Italy, Slovenia and Croatia.
Fabio M. Dalla Vecchia
doaj
Geologia Croatica, 2016 Early-intracontinental rifting of Pangea is result of thermal doming in Uppermost Permian time giving rise to the formation of horst-graben structures, followed by slow subsidence, marine transgression and evaporate deposition.
Ladislav Palinkaš +4 more
doaj +1 more source