Results 111 to 120 of about 7,272 (219)

The effect of carbonic anhydrase IX on focal contacts during cell spreading and migration [PDF]

, 2013
Carbonic anhydrase IX is a hypoxia-induced transmembrane enzyme linked with solid tumors. It catalyzes the reversible hydration of CO(2) providing bicarbonate ions for intracellular neutralization and protons for extracellular acidosis, thereby ...
Eliska eSvastova, Juraj eKopacek, Lucia eCsaderova, Lucia eCsaderova, Magdalena eVrestiakova, Matej eStano, Michaela eDebreova, Peter eRadvak, Silvia ePastorekova   +8 more
core   +2 more sources

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Recent Advances (2023–2025) of Capillary Electrophoresis‐Mass Spectrometry (CE‐MS) for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Top‐down proteomics (TDP) characterizes proteoforms in cells, tissues, and biofluids, in discovery mode and on a global scale, requiring analytical tools with high peak capacity for proteoform separation and high sensitivity for proteoform detection, given the extremely high proteoform complexity and wide proteoform concentration dynamic range.
Guijie Zhu, David A. Alvarez Rodriguez, Noah Gould, Alexander R. Ivanov, Kevin Jooß, Liangliang Sun   +5 more
wiley   +1 more source

Movement Disorders in Developmental and Epileptic Encephalopathies

Movement Disorders Clinical Practice, EarlyView.
Abstract Background Monogenic developmental and epileptic encephalopathies (DEE) frequently feature co‐occurring movement disorders. Gene discovery has expanded epilepsy‐dyskinesia syndromes (EDS) from classic associations such as stereotypies in Rett syndrome to PRRT2‐related infantile seizures with paroxysmal dyskinesia and crouched gait in SCN1A ...
Shekeeb Mohammad, Darius Ebrahimi‐Fakhari, Hugo Morales‐Briceno   +2 more
wiley   +1 more source

Migrasomes, Matrix‐Bound Nanovesicles, and More: Messengers in the Matrix

PROTEOMICS, EarlyView.
ABSTRACT Extracellular vesicles (EVs) and particles (EPs) are diverse micro‐ and nanoparticles that circulate in bodily fluids and can attach to, or be deposited onto, the extracellular matrix (ECM) and other surfaces. To date, the nomenclature and classification of matrix‐bound or matrix‐associated EVs and EPs (MEVPs) have been unclear, largely due to
Anna V. Kolesov, Natalie Reitz, Marley Dewey, Colin L. Hisey   +3 more
wiley   +1 more source

Catalytically inactive carbonic anhydrase‐related proteins enhance transport of lactate by MCT1

FEBS Open Bio, 2019
Carbonic anhydrases (CA) catalyze the reversible hydration of CO2 to protons and bicarbonate and thereby play a fundamental role in the epithelial acid/base transport mechanisms serving fluid secretion and absorption for whole‐body acid/base regulation ...
Ashok Aspatwar, Martti E. E. Tolvanen, Hans‐Peter Schneider, Holger M. Becker, Susanna Narkilahti, Seppo Parkkila, Joachim W. Deitmer   +6 more
doaj   +1 more source

Parallel Analysis of Acidic and Basic Proteoforms in Cell Lysates via Native Cation and Anion Exchange Chromatography—Native Mass Spectrometry

PROTEOMICS, EarlyView.
ABSTRACT Comprehensive characterization of proteoforms in complex biological systems remains a significant challenge. While native ion‐exchange chromatography (IEC) hyphenated with native mass spectrometry (nMS) is a powerful tool for resolving proteoforms, three critical limitations persist: restricted volatile buffers causing nonlinear pH gradients ...
Ziran Zhai, Hafsa Zakri, Matteo Damian, Francesco G. Mutti, Garry Corthals, Andrea F. G. Gargano   +5 more
wiley   +1 more source

Facile synthesis of aminobiphenyl sulfonamides via Chan–Lam coupling and their biological evaluation as potent carbonic anhydrase inhibitors

Scientific Reports
Inhibition of carbonic anhydrases (EC 4.2.1.1, hCAs) is known to be a potential target for treatment of several disorders like epilepsy, glaucoma, obesity, and cancer.
Moniba Sharif, Abid Mahmood, Aqsa Kanwal, Matloob Ahmad, Nasir Rasool, Muhammad Usman Qamar, Mohammed H. AL Mughram, Amira Ali Mohammed Al-Harethi, Tawaf Ali Shah, Imran Ibrahim Shaikh   +9 more
doaj   +1 more source

Gene turnover in the common ancestor of all C4 grasses

PLANTS, PEOPLE, PLANET, EarlyView.
Understanding how plants evolve more efficient photosynthesis is important in a warming world where improving crop productivity and resilience is a global priority. By generating the first reference genomes for an early‐diverging group of grasses called the Aristidoideae, we were able to reconstruct the genetic makeup of the last common ancestor of all
Lara Pereira, Ahmed S. Alenazi, Sahr Mian, Ilia J. Leitch, Pascal‐Antoine Christin, Colin P. Osborne, Luke T. Dunning   +6 more
wiley   +1 more source

A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity

Nature Communications
Stomata of plant leaves open to enable CO2 entry for photosynthesis and close when CO2 in the leaf is elevated. CO2 is thought to promote stomatal closure in part by activating the SLAC1 anion channel at the guard cell plasma membrane.
Lingfeng Xia, Jonas Chaves Alvim, Thanh-Hao Nguyen, Cecile Lefoulon, Fernanda A. L. Silva-Alvim, Zhiyi Yu, Martina Klejchova, Sahar Farami, Sakharam Waghmare, Rucha Karnik, Michael R. Blatt   +10 more
doaj   +1 more source