Results 141 to 150 of about 14,405 (167)
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Molecular Cancer Therapeutics, 2004
Abstract Carboxylesterases, expressed at high levels in human liver and intestine, are thought to detoxify xenobiotics. The anticancer prodrug 7-ethyl-10-[4-1-piperidino)-1-piperidino]carbonyloxycamptothecin (CPT-11) is also metabolized by carboxylesterases to produce the active drug 7-ethyl-10-hydroxycamptothecin.
Kyoung Jin P, Yoon +5 more
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Abstract Carboxylesterases, expressed at high levels in human liver and intestine, are thought to detoxify xenobiotics. The anticancer prodrug 7-ethyl-10-[4-1-piperidino)-1-piperidino]carbonyloxycamptothecin (CPT-11) is also metabolized by carboxylesterases to produce the active drug 7-ethyl-10-hydroxycamptothecin.
Kyoung Jin P, Yoon +5 more
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Carboxylesterase from Sulfolobus solfataricus P1
2001Publisher Summary To date, relatively few investigations regarding the purification and characterization of thermostable (T opt > 60 °) esterases have been conducted. Thus far, esterases from Bacillus acidocaldarius, Pyrococcus furiosus , Bacillus stearothermophilus , Sulfolobus shibatae , Thermoanaerobacterium sp, Pyrococcus abyssi , and ...
A C, Sehgal +4 more
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2020
This chapter focuses on important issues in the design and development of prodrugs such as a different substrate specificities of human carboxylesterase isozymes and other hydrolases. Prodrugs such as acemetacin and indomethacin farnesyl have been developed to reduce the side effects of indomethacin. The prodrug enol-esterified with pivalic acid has an
Masato Takahashi, Masakiyo Hosokawa
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This chapter focuses on important issues in the design and development of prodrugs such as a different substrate specificities of human carboxylesterase isozymes and other hydrolases. Prodrugs such as acemetacin and indomethacin farnesyl have been developed to reduce the side effects of indomethacin. The prodrug enol-esterified with pivalic acid has an
Masato Takahashi, Masakiyo Hosokawa
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Canadian Journal of Biochemistry, 1975
Chicken, sheep, and horse liver carboxylesterases have been purified by procedures involving ammonium sulfate fractionation, ion-exchange chromatography and gel filtration on Sephadex. The actual yields of the procedures described were as follows: chicken, 1 g from 2 kg of liver powder (chloroform–acetone); sheep, 200 mg from 400 g of powder ...
P A, Inkerman +5 more
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Chicken, sheep, and horse liver carboxylesterases have been purified by procedures involving ammonium sulfate fractionation, ion-exchange chromatography and gel filtration on Sephadex. The actual yields of the procedures described were as follows: chicken, 1 g from 2 kg of liver powder (chloroform–acetone); sheep, 200 mg from 400 g of powder ...
P A, Inkerman +5 more
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Journal of Biochemistry, 2010
A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2).
David M, Charbonneau +2 more
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A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2).
David M, Charbonneau +2 more
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Biochemistry, 1969
The molecular weight of pig liver carboxylesterase (~88% pure) has been determined as 163,000 (±15,000). The enzyme has two active sites per molecular weight of 163,000 as shown by titration with pnitrophenyl dimethylcarbamate and p-nitrophenyl diethyl phosphate.
Horgan, Douglas J. +4 more
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The molecular weight of pig liver carboxylesterase (~88% pure) has been determined as 163,000 (±15,000). The enzyme has two active sites per molecular weight of 163,000 as shown by titration with pnitrophenyl dimethylcarbamate and p-nitrophenyl diethyl phosphate.
Horgan, Douglas J. +4 more
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Measurement of Carboxylesterase (
AbstractMeasurement of Carboxylesterase (CES) Activities (Masakiyo Hosokawa, Chiba University, Chiba, Japan and Tetsuo Satoh, Biomedical Research Institute, Chiba, Japan). Mammalian carboxyesterase (CES) efficiently catalyzes the hydrolysis of a variety of ester‐ and amide‐containing chemicals.
Masakiyo, Hosokawa, Tetsuo, Satoh
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Tissue carboxylesterase activity of rainbow trout
Environmental Toxicology and Chemistry, 1999AbstractThe activity of carboxylesterase (CaE), a class of nonspecific serine hydrolases, was evaluated in vitro in tissues and microsomes of rainbow trout and compared to esterase activity in rats, other fish species, and embryo to adult life stages of trout. Trout gill and liver microsomes exhibited substantial CaE activity and limited variation over
Mace G. Barron +3 more
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Carboxylesterases (EC 3.1.1). Dissociation of ox liver carboxylesterase
Biochemistry, 1969M T, Runnegar, E C, Webb, B, Zerner
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Studies on liver carboxylesterases
2017Procedures for the purification of horse and shark liver carbostyl.esterases are reported together with a modified procedure for the purification of sheep liver carboxylesterase. In the procedure for the horse enzyme, the starting material is an acetone powder, whereas for the other two enzymes, it is a chloroform-acetone powder.
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