Results 351 to 360 of about 1,441,839 (397)

How to isolate cardiac myocytes

Cardiovascular Research, 1994
A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of ...
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Ephaptic Coupling in Cardiac Myocytes

IEEE Transactions on Biomedical Engineering, 2013
While it is widely believed that conduction in cardiac tissue is regulated by gap junctions, recent experimental evidence suggests that the extracellular space may play a significant role in action potential propagation. Cardiac tissue with low gap junctional coupling still exhibits conduction, with conflicting degrees of slowing that may be due to ...
Joyce, Lin, James P, Keener
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Confocal Microscopy of Cardiac Myocytes

2013
Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue.
Robert L, Price, Stephen T, Haley, Tara, Bullard, Jeffrey, Davis, Thomas K, Borg, Louis, Terracio   +5 more
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Scaffolding Proteins in Cardiac Myocytes

2008
Post-translational modification, such as protein phosphorylation, plays a critical role to reversibly amplify and modulate signaling pathways. Since kinases and phosphatases have broad substrate recognition motifs, compartmentalization and localization of signaling complexes are required to achieve specific signals.
N L, Chudasama, S O, Marx, S F, Steinberg   +2 more
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Cardiac myocytes release leukocyte-stimulating factors

American Journal of Physiology-Heart and Circulatory Physiology, 1995
The production of cytokines directly from cardiac myocytes has not been previously demonstrated and could represent an important mechanism and site of intervention in ischemia and reperfusion injuries. Macrophage inflammatory protein-2 (MIP-2) and monocyte chemotactic protein (MCP) are chemotactic cytokines (chemokines) that stimulate ...
K D, Massey, R M, Strieter, S L, Kunkel, J M, Danforth, T J, Standiford   +4 more
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Cardiac myocyte guanosine transport and metabolism

American Journal of Physiology-Cell Physiology, 1987
Guanosine transport and metabolism were examined in adult rat cardiac myocytes. Myocytes transported guanosine via saturable [Km = 18 microM, maximum velocity (Vmax) = 3.61 pmol.mg-1.s-1] and nonsaturable (rate constant = 1.47 X 10(-2] processes. The saturable process was inhibited by nitrobenzyl-thioinosine, inosine [inhibition constant (Ki) = 180 ...
T P, Geisbuhler, D A, Johnson, M J, Rovetto   +2 more
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Anoxic injury of adult cardiac myocytes

1984
Cultured adult cardiocytes were exposed to anoxia. The initial decrease of high-energy phosphates was accompanied by a moderate release of cytosolic enzymes and morphological changes: the appearance of sarcolemmal 'microblebs' (approximately 1 micron in diameter) and an increase of subsarcolemmal vesicles. At ATP levels above 2 mumol/gww, metabolic and
H M, Piper, P, Schwartz, R, Spahr, J F, Hütter, P G, Spieckermann   +4 more
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Oxygen Transport to Ischemic Cardiac Myocytes

1997
Po2 at mitochondrial innermembrane is determined by capillary blood Po2 and Po2 gradients between these two sites. The Po2 gradient of actively metabolizing tissue such as beating heart is considerably higher. Consequently, intracellular Po2 of the normal heart in situ may be as low as P50 of cytosolic myoglobin (2 ~ 5 Torr) (Coburn et al., 1973 ...
E, Takahashi, K, Doi
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Commitment and differentiation of cardiac myocytes

Trends in Cardiovascular Medicine, 1992
This article reviews what is known about the earliest stages of heart development focusing on the periods of commitment and differentiation of cardiac progenitor cells and their molecular regulation. The pathway from precursor to differentiated cardiac myocyte is crucial to forming a normal, functional heart.
J, Litvin, M, Montgomery, A, Gonzalez-Sanchez, J G, Bisaha, D, Bader   +4 more
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Adrenergic regulation of cardiac myocyte apoptosis

Journal of Cellular Physiology, 2001
AbstractThe direct effects of catecholamines on cardiac myocytes may contribute to both normal physiologic adaptation and pathologic remodeling, and may be associated with cellular hypertrophy, apoptosis, and alterations in contractile function.
K, Singh, L, Xiao, A, Remondino, D B, Sawyer, W S, Colucci   +4 more
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