Results 21 to 30 of about 537 (137)

Cardosins in postembryonic development of cardoon: towards an elucidation of the biological function of plant aspartic proteinases [PDF]

Protoplasma, 2008
Following on from previous work, the temporal and spatial accumulation of the aspartic proteinases (EC 3.4.23) cardosin A and cardosin B during postembryonic seed development of cardoon (Cynara cardunculus) was studied, mRNA and protein analyses of both cardosins suggested that the proteins accumulate during seed maturation, and that cardosin A is ...
Pereira, Cláudia Sofia, Costa, Diana Soares da, Pereira, Susana, Nogueira, F. de Moura, Albuquerque, P. M., Teixeira, J., Faro, C., Pissarra, J.   +7 more
openaire   +5 more sources

Cardosin A Molecular Determinants and Biosynthetic Pathways

, 2012
Déterminants moléculaires et voies de synthèse de la cardosine A La cardosine A est une protéase aspartique identifiée il y a plus de 20 ans dans les cellules du chardon Cynara cardunculus. Sa distribution dans tous les tissus de la plante et ses caractéristiques enzymatiques ont été caractérisées par approches biochimiques.
Pereira, Cláudia
openaire   +3 more sources

Molecular Characterization of Peptides Released from β-Lactoglobulin and α-Lactalbumin via Cardosins A and B [PDF]

Journal of Dairy Science, 2006
Crude mixtures of aspartic proteases from flowers of the plant Cynara cardunculus have been studied frequently, as have activities of such enzymes (in pure form) on caseins from bovine, ovine, and caprine sources. This research study addressed pure bovine whey protein as substrates; that is, alpha-lactalbumin (alphaLA) and beta-lactoglobulin (alpha-LG),
Barros, R. M., Malcata, F. X.
openaire   +5 more sources

Production of Plant Proteases and New Biotechnological Applications: An Updated Review. [PDF]

ChemistryOpen, 2022
Plant proteases are increasingly used in industrial applications. Potential application fields are continuously broadening, ranging from the production of bioactive peptides to the treatment of liquid effluents, including the recovery of by‐products with high protein content and many other interesting applications which are reviewed.
David Troncoso F, Alberto Sánchez D, Luján Ferreira M.   +2 more
europepmc   +2 more sources

Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localisation. [PDF]

Plant Biotechnol J
ABSTRACT Plant systems have gained increased attention as an alternative platform for producing heterologous proteins, particularly for industrially relevant proteins. The Cynara cardunculus L. flower extract is traditionally used in cheese production across Mediterranean countries due to its milk‐clotting properties.
Muthusamy S, Vetukuri RR, Lundgren A, Kim S, Kalyandurg PB, Strid Å, Zhu LH, Kanagarajan S, Brodelius PE.   +8 more
europepmc   +2 more sources

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Advanced Science, Volume 9, Issue 3, January 25, 2022., 2022
Cultivating meat from cells is a promising solution to the environmental, ethical, health, and food‐security challenges associated with conventional meat production. Cultivated meat will require the development of scalable, low‐cost, and edible or biodegradable scaffolds to support cell growth.
Claire Bomkamp, Stacey C. Skaalure, Gonçalo F. Fernando, Tom Ben‐Arye, Elliot W. Swartz, Elizabeth A. Specht   +5 more
wiley   +1 more source

Milk‐Gelling Properties of Proteases Extracted from the Fruits of Solanum Elaeagnifolium Cavanilles

International Journal of Food Science, Volume 2022, Issue 1, 2022., 2022
There is little information on the milk coagulation process by plant proteases combined with chymosins. This work is aimed at studying the capability of protease enclosed in the ripe fruits of Solanum elaeagnifolium (commonly named trompillo) to form milk gels by itself and in combination with chymosin.
Carolina Nájera-Domínguez, Néstor Gutiérrez-Méndez, Diego E. Carballo-Carballo, María Rosario Peralta-Pérez, Blanca Sánchez-Ramírez, Guadalupe Virginia Nevarez-Moorillón, Armando Quintero-Ramos, Antonio García-Triana, Efren Delgado, CHANDRA MOHAN CHANDRA SEKAR   +9 more
wiley   +1 more source

Activation, Proteolytic Processing, and Peptide Specificity of Recombinant Cardosin A [PDF]

Journal of Biological Chemistry, 2005
Cardosins are model plant aspartic proteases, a group of proteases that are involved in cell death events associated with plant senescence and stress responses. They are synthesized as single-chain zymogens, and subsequent conversion into two-chain mature enzymes is a crucial step in the regulation of their activity. Here we describe the activation and
Pedro, Castanheira, Bart, Samyn, Kjell, Sergeant, José C, Clemente, Ben M, Dunn, Euclides, Pires, Jozef, Van Beeumen, Carlos, Faro   +7 more
openaire   +2 more sources

Camel Milk‐Clotting Using Plant Extracts as a Substitute to Commercial Rennet

Journal of Chemistry, Volume 2021, Issue 1, 2021., 2021
The transformation of camel milk into cheese is an operation considered very delicate because of several difficulties encountered in achieving coagulation. The present study aims to improve coagulation abilities of camel milk using enzyme extracts from pineapple, kiwi, and ginger.
Imen Fguiri, Moufida Atigui, Amel Sboui, Arroum Samira, Chayma Marzougui, Mohamed Dbara, Mohamed Hammadi, Touhami Khorchani, Murat Senturk   +8 more
wiley   +1 more source

Cardosins: A new and efficient plant enzymatic tool to dissociate neuronal cells for the establishment of cell cultures [PDF]

Biotechnology and Bioengineering, 2007
AbstractIn the present work, we examined the feasibility of using cardosins, plant aspartic‐proteinases from Cynara cardunculus L., to isolate cells from rat embryonic brain. Using morphological and functional assays, we compared cell cultures obtained with cardosins with those prepared with a well‐established trypsin protocol.
Duarte, A. S., Rosa, N., Duarte, E. P., Pires, E., Barros, M. T.   +4 more
openaire   +3 more sources