Journal of Botany, Volume 2010, Issue 1, 2010., 2010 Etiolation of vegetables is a complex phenomenon implying qualitative and quantitative protein changes. The 2‐DE protein profile of green and etiolated fleshy stalk samples has shown great differences in the 4–7 pH and 10 to 250 kDa mass range. Currently, a shortage of Cynara DNA or protein sequences has required the use of de novo sequencing and BLAST
Carmine Guarino +8 more
wiley +1 more source
Cardosins improve neuronal regeneration after cell disruption: a comparative expression study
Cell Biology and Toxicology, 2008 The establishment of primary cell cultures is invaluable for studying cell and molecular biological questions. Although primary cell cultures more closely resemble and function like in the native environment, during the culture establishment the cells undergo several changes including the damage sustained during their removal from original tissue.
Duarte, Ana +4 more
openaire +4 more sources
Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. [PDF]
, 2007 Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese.
Pimentel, C +11 more
core +1 more source
Purification, Characterization and Partial Amino Acid Sequencing of Two New Aspartic Proteinases from Fresh Flowers of Cynara cardunculus L. [PDF]
, 1996 Two new aspartic proteinases have been isolated from stigmas of the cardoon Cynara cardunculus L. by a two-step purification procedure including extraction at low pH, gel filtration on Superdex 200, and ion-exchange chromatography on Mono Q.
Tang, Jordan +5 more
core +1 more source
The Glycosylation of the Aspartic Proteinases from Barley (Hordeum Vulgare L.) and Cardoon (Cynara Cardunculus L.) [PDF]
, 1997 Plant aspartic proteinases characterised at the molecular level contain one or more consensus N-glycosylation sites [Runeberg-Roos, P., Törmäkangas, K. & Östman, A. (1991) Eur. J. Biochem. 202, 102120131027; Asakura, T., Watanabe, H., Abe, K. & Arai, S. (
Costa, Júlia +23 more
core +1 more source
, 2013 International audience: Several vacuolar sorting determinants (VSDs) have been described for protein trafficking to the vacuoles in plant cells. Because of the variety in plant models, cell types and experimental approaches used to decipher vacuolar ...
Pereira, Susana +3 more
core +1 more source
Identification and proteolytic processing of procardosin A [PDF]
, 1998 Plant aspartic proteinases contain a plant-specific insert (PSI) of about 100 amino acids of unknown function with no similarity with the other aspartic proteinases but with significant similarity with saposins, animal sphingolipid activator proteins ...
Beeumen, Jozef Van +7 more
core +1 more source
Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
, 2022 Cynara cardunculus L. flowers have been used, in the form of an aqueous extract as vegetable coagulant, in the production of Protected Designation of Origin (PDO) ewe’s cheeses in the Mediterranean Basin.
Fonseca, Ana +3 more
core
Selected Cardoon (Cynara cardunculus L.) Genotypes Suitable for PDO Cheeses in Mediterranean Regions [PDF]
, 2018 Cardoon flower extract is a traditional and exclusive rennet used for some PDO cheeses in several Mediterranean regions, due to its extremely high concentration in cardosins.
Euclides Pires +7 more
core +1 more source
Multiplicity of aspartic proteinases from Cynara cardunculus L.
, 2009 Aspartic proteinases (AP) play major roles in physiologic and pathologic scenarios in a wide range of organisms from vertebrates to plants or viruses.
Domingues, M Rosario M +12 more
core +1 more source