Results 211 to 220 of about 251,083 (336)

Prevention of muscular dystrophy in mice by CRISPR/Cas9–mediated editing of germline DNA

, 2014
Chengzu Long, John McAnally, John M. Shelton, Alex A. Mireault, Rhonda Bassel‐Duby, Eric N. Olson   +5 more
openalex   +1 more source

Protein Engineering of Cas9 for Enhanced Function [PDF]

, 2014
Benjamin L. Oakes, Dana C. Nadler, David F. Savage   +2 more
openalex   +1 more source

Gastrula‐Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross‐Talk with TGF‐β Signaling Pathway

Advanced Science, EarlyView.
A gastrula‐premarked enhancer within the first intron of Cdx2 governs posterior embryonic development via both regulating Cdx2 expression in cis and coordinating TGF‐β signaling in trans to ensure proper posterior body development. Functional rescue in gastruloids reveals a Cdx2‐independent pathway, highlighting p‐Enh‐eRNA as key epigenetic ...
Yingying Chen, Fengxiang Tan, Qing Fang, Lin Zhang, Jiaoyang Liao, Penglei Shen, Yun Qian, Mingzhu Wen, Rui Song, Yonggao Fu, He Jax Xu, Ran Wang, Cheng Li, Zhen Shao, Jinsong Li, Naihe Jing, Xianfa Yang   +16 more
wiley   +1 more source

CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability. [PDF]

GM Crops Food
Zeng Z, Wang H, Luo Y, Chen W, Xu M, Wei H, Chen Z, Xiang T, Wang L, Han N, Huang X, Bian H.   +11 more
europepmc   +1 more source

Creating Cancer Translocations in Human Cells Using Cas9 DSBs and nCas9 Paired Nicks [PDF]

, 2014
Benjamin Renouf, Marion Piganeau, Hind Ghezraoui, Maria Jasin, Erika Brunet   +4 more
openalex   +1 more source

In Vivo Cytosolic Delivery of Biomolecules into Neurons for Super‐Resolution Imaging and Genome Modification

Advanced Science, EarlyView.
The N1 peptide specifically targets neurons, enabling cytosolic delivery of fluorescent dyes and proteins for super‐resolution imaging and functional genetic modification. Abstract Efficient delivery of biomolecules into neurons has significant impacts on therapeutic applications in the central nervous system (CNS) and fundamental neuroscience research.
Xiaoqian Ge, Joseph B. Wekselblatt, Scott Elmore, Bo Wang, Tongtong Wang, Renjinming Dai, Tingting Zhang, Harsh Dave, Mohammadaref Ghaderi, Athul Raj Anilkumar, Bill Wang, Shashank R. Sirsi, Jung‐Mo Ahn, Mikhail G. Shapiro, Yuki Oka, Carlos Lois, Zhenpeng Qin   +16 more
wiley   +1 more source

Non-variable RNA deletion using the CRISPR-Cas9 technique demonstrated improved outcomes in human intestine single-cell RNA sequencing data, even at half sequencing depths. [PDF]

Genomics Inform
Kim DJ, Joh CSY, Jeong SY, Kim YJ, Koh SJ, Kim HJ.   +5 more
europepmc   +1 more source

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9

, 2014
Hongmei Lisa Li, Naoko Fujimoto, Noriko Sasakawa, Saya Shirai, Tokiko Ohkame, Tetsushi Sakuma, Michihiro Tanaka, Naoki Amano, Akira Watanabe, Hidetoshi Sakurai, Takashi Yamamoto, Shinya Yamanaka, Akitsu Hotta   +12 more
openalex   +1 more source

Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas9 [PDF]

, 2014
Taichi Matsunaga, Jun K. Yamashita
openalex   +1 more source

Neutrophil Macrophage Crosstalk via Extracellular Vesicles Drives Reverse Migration in a Fully Human Model of Wound Healing

Advanced Science, EarlyView.
A novel microphysiological system that can mimic sterile or non‐sterile injury demonstrates that macrophage‐derived extracellular vesicles modulate neutrophil migratory responses to these different wound states. The model showed that resolution of inflammation through neutrophil reverse migration is driven by IL‐8 derived from extracellular vesicles ...
Kehinde Adebayo Babatunde, Oluwadamilola Fatimat Babatunde, Adeel Ahmed, Wilmara Salgado‐Pabon, David J Beebe, Sheena C. Kerr   +5 more
wiley   +1 more source