Results 311 to 320 of about 251,083 (336)
Assessment and Mitigation of CRISPR-Cas9-Induced Nontargeted Translocations. [PDF]
Adv Sci (Weinh)Hou Z +6 more
europepmc +1 more source
Specific macrophage RhoA targeting CRISPR-Cas9 for mitigating osteoclastogenesis-induced joint damage in inflammatory arthritis. [PDF]
Cell Rep MedChen J +13 more
europepmc +1 more source
Optimization of SgRNA expression with RNA pol III regulatory elements in Anopheles stephensi. [PDF]
Sci RepGonzalez E +7 more
europepmc +1 more source
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2023
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
Current Opinion in Biotechnology, 2017
Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Florian, Richter +5 more
openaire +2 more sources
Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Florian, Richter +5 more
openaire +2 more sources
Nature Biotechnology, 2015
Cpf1 is one of a growing number of class II CRISPR-Cas effectors that expand both our understanding of bacterial immunity and our genome-editing toolset.
Scot A. Wolfe, Erik J. Sontheimer
openaire +3 more sources
Cpf1 is one of a growing number of class II CRISPR-Cas effectors that expand both our understanding of bacterial immunity and our genome-editing toolset.
Scot A. Wolfe, Erik J. Sontheimer
openaire +3 more sources
2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources