Results 321 to 330 of about 251,083 (336)
Some of the next articles are maybe not open access.
Nature Methods, 2015
Two research groups show that the CRISPR system can be regulated by heterodimerizing a split Cas9.
openaire +3 more sources
Two research groups show that the CRISPR system can be regulated by heterodimerizing a split Cas9.
openaire +3 more sources
Liposomal delivery of CRISPR/Cas9 [PDF]
Cancer Gene Therapy, 2019 Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The surface properties of liposomal carriers, including the surface charge, PEGylation, and ligand modification can significantly affect the gene silencing efficiency. Three barriers of systemic CRISPR/Cas9 delivery (long blood circulation, efficient tumor penetration,
Xu Li, Xu Li, Shuai Zhen, Shuai Zhen
openaire +2 more sources
MicroRNAs tame CRISPR–Cas9 [PDF]
Nature Cell Biology, 2019 MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina Jouravleva, Phillip D. Zamore
openaire +2 more sources
Nature, 2016
The enzyme Cas9 is used in genome editing to cut selected DNA sequences, but it also creates breaks at off-target sites. Protein engineering has now been used to make Cas9 enzymes that have minimal off-target effects. See Article p.490 The CRISPR–Cas9 nucleases now widely used in gene editing can be
openaire +3 more sources
The enzyme Cas9 is used in genome editing to cut selected DNA sequences, but it also creates breaks at off-target sites. Protein engineering has now been used to make Cas9 enzymes that have minimal off-target effects. See Article p.490 The CRISPR–Cas9 nucleases now widely used in gene editing can be
openaire +3 more sources
PrePAIRing Cas9s for screening success
Nature Biotechnology, 2018Orthogonal CRISPR–Cas9 nucleases enable more efficient high-throughput screens for deciphering genetic interactions.
Wensheng Wei, Zhuo Zhou
openaire +3 more sources
2018
In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob Blæsbjerg Hoof +2 more
openaire +2 more sources
In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob Blæsbjerg Hoof +2 more
openaire +2 more sources
CRISPR–Cas9 Structures and Mechanisms
Annual Review of Biophysics, 2017Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Fuguo Jiang, Jennifer A. Doudna
openaire +2 more sources
CRISPR Cas9 − Licensing the unlicensable
Journal of Biotechnology, 2018A new gene engineering technology has recently made it through the media, not only because of its technical advantages, but also because it is in the focus of an epic patent battle between two academic institutions. The technology bears the cryptic name "CRISPR Cas9", and allows the manipulation of genes (so called "gene editing") with so far unseen ...
openaire +3 more sources
Nature Reviews Molecular Cell Biology, 2020
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
openaire +3 more sources
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
openaire +3 more sources
CRISPR/Cas9 in the Chicken Embryo
2017Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms.
Nadège Véron +2 more
openaire +3 more sources