Results 61 to 70 of about 850,147 (309)

Heterologous Expression and Enzymatic Properties of β-Glucuronidase from Clostridium perfringens and Its Application in Bilirubin Transformation

Microorganisms
β-glucuronidase is an important hydrolase, which plays an important role in drug metabolism, clinical diagnostics, and biotransformation. This study focuses on the heterologous expression, isolation, purification, and its enzymatic properties of β ...
Qianlin Wu, Qing Guo, Fo Yang, Mengru Li, Yumeng Zhu, Binpeng Xu, Lu Zhao, Shanshan Zhang, Youyu Xie, Feng Li, Xiaomin Wu, Dayong Xu   +11 more
doaj   +1 more source

Magnetoelectrically driven catalytic degradation of organics

, 2019
Here, we report the catalytic degradation of organic compounds by exploiting the magnetoelectric (ME) nature of cobalt ferrite-bismuth ferrite (CFO-BFO) core-shell nanoparticles.
Chen, Xiang-Zhong, Hoop, Marcus, Limburg, Gregory, Marti, Xavi, Mushtaq, Fajer, Nelson, Bradley J., Pané, Salvador, Siringil, Erdem Can, Steuer, Christian, Stipp, Patrick, Torlakcik, Harun   +10 more
core   +1 more source

Substrate-Assisted Catalysis Unifies Two Families of Chitinolytic Enzymes [PDF]

, 1997
Hen egg-white lysozyme has long been the paradigm for enzymatic glycosyl hydrolysis with retention of configuration, with a protonated carboxylic acid and a deprotonated carboxylate participating in general acid-base catalysis.
Blake C. C. F., C−, Davies G. J., Ford L. O., Henrissat B., Iseli B., Johnson L. N., Jones T. A., Kirby A., Knapp S., Kobayashi S., Koshland D. E., Perrakis A., Phillips D. C., Piszkiewicz D., Rao V. H., Rozeboom H. J., Sinnot M. L., Strynadka N. C. J., Sulzenbacher G., Terwisscha van Scheltinga A. C., Terwisscha van Scheltinga A. C., Terwisscha van Scheltinga A. C., Tews I., Thunnissen A. M. W. H., Tronrud D. E., van Roey P., Vårum K. M., Weaver L. H.   +28 more
core   +3 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural Insights into Arginine Kinase and Phosphagen Kinase Homologs: Mechanisms of Catalysis, Regulation, and Evolution

Biology
Phosphagen kinases are vital for energy buffering and ATP regeneration in cells with high or fluctuating energy demands. Phosphagens are small, high-energy phosphate-storage molecules, such as arginine phosphate or creatine phosphate, that serve as ...
Sung-Min Kang
doaj   +1 more source

Theoretical identification of the interactions between the zeolite framework and the hydrocarbon pool co-catalyst in methanol-to-olefin conversion [PDF]

, 2009
The rapidly increasing demand of oil-based chemicals calls for the development of new technologies based on other natural sources. Among these emerging alternatives, the methanol-to-olefin process (MTO) in acidic zeolites is one of the most promising ...
Lesthaeghe, David, Van Speybroeck, Veronique, Waroquier, Michel   +2 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Insights into the mechanism of substrate specificity in a novel PL15_3 subfamily oligo-alginate lyase VBAly15A

Applied and Environmental Microbiology
Alginate is a major component of brown algae cell walls and can be degraded via β-elimination by alginate lyases. These enzymes are classified into polysaccharide lyases and oligo-alginate lyases (Oals), with Oals mainly represented by the PL15 and PL17 ...
Yongqi Tang, Ziyan Song, Xiaodong Xu, Yingjie Li, Lushan Wang   +4 more
doaj   +1 more source

Finding a suitable catalyst for on-board ethanol reforming using exhaust heat from an internal combustion engine [PDF]

, 2017
Ethanol steam reforming with pure ethanol and commercial bioethanol (S/C = 3) was carried out inside the housing of the exhaust gas pipe of a gasoline internal combustion engine (ICE) by using exhaust heat (610–620 °C).
Bosch Tous, Ricardo, Casanovas Grau, Albert, Jiménez Divins, Nuria, Llorca Piqué, Jordi, Rejas García, Albert   +4 more
core   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source