Results 41 to 50 of about 10,401,391 (311)

Metallo-β-Lactamase Inhibitors Inspired on Snapshots from the Catalytic Mechanism

Biomolecules, 2020
β-Lactam antibiotics are the most widely prescribed antibacterial drugs due to their low toxicity and broad spectrum. Their action is counteracted by different resistance mechanisms developed by bacteria.
Antonela R. Palacios, M. Rossi, Graciela Mahler, A. Vila   +3 more
semanticscholar   +1 more source

Elucidating the catalytic mechanism of Prussian blue nanozymes with self-increasing catalytic activity

Nature Communications
Although Prussian blue nanozymes (PBNZ) are widely applied in various fields, their catalytic mechanisms remain elusive. Here, we investigate the long-term catalytic performance of PBNZ as peroxidase (POD) and catalase (CAT) mimetics to elucidate their ...
Kaizheng Feng, Zhenzhen Wang, Shi Wang, Guancheng Wang, Haijiao Dong, Hongliang He, Haoan Wu, Ming Ma, Xing-Fa Gao, Yu Zhang   +9 more
semanticscholar   +1 more source

Confluence of Theory and Experiment Reveal the Catalytic Mechanism of the Varkud Satellite Ribozyme

Nature Chemistry, 2020
The Varkud satellite ribozyme catalyses site-specific RNA cleavage and ligation, and serves as an important model system to understand RNA catalysis. Here, we combine stereospecific phosphorothioate substitution, precision nucleobase mutation and linear ...
Abir Ganguly, Benjamin P. Weissman, Timothy J. Giese, Nan-Sheng Li, Shuichi Hoshika, Saieesh A. Rao, S. Benner, J. Piccirilli, D. York   +8 more
semanticscholar   +1 more source

Variety in the USP deubiquitinase catalytic mechanism

Life Science Alliance
Quantitative analysis shows that USP family members have varying catalytic triads. Their third and fourth catalytic residues change in role and importance for nucleophilic attack and catalysis.
Niels Keijzer, A. Priyanka, Yvette Stijf-Bultsma, Alexander Fish, M. Gersch, T. Sixma   +5 more
semanticscholar   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

In Situ Raman Study of Layered Double Hydroxide Catalysts for Water Oxidation to Hydrogen Evolution: Recent Progress and Future Perspectives

Energies
With the increasing global emphasis on green energy and sustainable development goals, the electrocatalytic oxygen evolution reaction (OER) is gradually becoming a crucial focus in research on water oxidation for hydrogen generation.
Jiafeng Wen, Siyuan Tang, Xiang Ding, Yin Yin, Fuzhan Song, Xinchun Yang   +5 more
doaj   +1 more source

Research progress on enhancement of bacterial α-amylase activity

Siliao yanjiu, 2023
α-amylase is an endonuclease that acts on the α-1,4-glucoside bond of starch molecules to reduce starch viscosity. As a commonly used additive in animal feed, α-amylase can make up for the deficiency of animal amylase, accelerate the starch ...
BAI Jing, WANG Jun, LI Mo, SONG Li-li
doaj  

Structural insights into FTO’s catalytic mechanism for the demethylation of multiple RNA substrates

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance The RNA modification N6-methyladenosine (m6A) was the first physiological substrate of FTO to be discovered. Recently, cap N6,2′-O-dimethyladenosine (m6Am), internal m6Am, and N1-methyladenosine were also found to be physiological substrates
Xiao Zhang, Lian-Huan Wei, Yuxin Wang, Yu Xiao, Jun Liu, Wei Zhang, Ning Yan, Gubu Amu, Xinjing Tang, Liang Zhang, G. Jia   +10 more
semanticscholar   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source