Results 261 to 270 of about 240,202 (291)

Mitochondrial Targeting of Catalytic RNAs

2015
Genetic transformation of mitochondria in multicellular eukaryotes has remained inaccessible, hindering fundamental investigations and applications to gene therapy or biotechnology. In this context, we have developed a strategy to target nuclear transgene-encoded RNAs into mitochondria in plants.
Daria, Mileshina, Adnan Khan, Niazi, Eliza, Wyszko, Maciej, Szymanski, Romain, Val, Clarisse, Valentin, Jan, Barciszewski, André, Dietrich   +7 more
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In vitro selection of catalytic RNAs

Current Opinion in Structural Biology, 1994
In vitro selection techniques are poised to allow a rapid expansion of the study of catalysis by RNA enzymes (ribozymes). This truly molecular version of genetics has already been applied to the study of the structures of known ribozymes and to the tailoring of their catalytic activity to meet specific requirements of substrate specificity or reaction
K B, Chapman, J W, Szostak
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Catalytic RNA: Structure and Mechanism

Biochemical Society Transactions, 1993
The Hopkins Lecturer is given the charge of explaining how advances in other fields of science have had an impact on his or her own field of biochemistry. A moment’s thought revealed that it was not at all difficult to think of examples where other scientific fields have provided necessary groundwork for the study of RNA catalysis or RNA biochemistry ...
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The Catalytic Mechanism of RNA Polymerase II

Journal of Chemical Theory and Computation, 2011
Eukaryotic RNA polymerase II (RNAP II) transcribes the DNA into mRNA. The presence of two metal ions (usually Mg(2+)) and conserved aspartate residues in the active sites of all nucleic acid polymerases led to the adoption of a universal catalytic mechanism, known as the "two metal ion catalysis".
Alexandra T P, Carvalho, Pedro A, Fernandes, Maria J, Ramos   +2 more
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Antisense Catalytic RNAs as Therapeutic Agents

1994
Publisher Summary This chapter presents an overview of well-characterized ribozymes with an emphasis on the problems that remain to be overcome before ribozyme therapy can be made a useful form of medical treatment. The chapter illustrates that many techniques are currently being used to enhance ribozyme activity.
D, Castanotto, J J, Rossi, N, Sarver
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Human catalytic RNA- and DNA-hydrolyzing antibodies

Journal of Immunological Methods, 2002
In patients with autoimmune diseases, anti-idiotypic antibodies directed to nucleoprotein complexes, DNA, and enzymes that participate in nucleic acid metabolism may be induced spontaneously by primary antigens and can have characteristics of the primary antigen, including catalytic activity.
Georgy A, Nevinsky, Valentina N, Buneva
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Transition State Stabilization by a Catalytic RNA

Science, 2002
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate–hairpin ribozyme complex with structures of precursor ...
Peter B, Rupert, Archna P, Massey, Snorri Th, Sigurdsson, Adrian R, Ferré-D'Amaré   +3 more
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The structure and function of catalytic RNAs

Science in China Series C: Life Sciences, 2009
Before the discovery of ribozymes, RNA had been proposed to function as a catalyst, based on the discovery that RNA folded into high-ordered structures as protein did. This hypothesis was confirmed in the 1980s, after the discovery of Tetrahymena group I intron and RNase P ribozyme.
QiJia, Wu, Lin, Huang, Yi, Zhang
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Coliphage Qβ RNA replication: RNA catalytic for single-strand release

Virology, 1991
We have generated 14 recombinant RNA templates for Q beta replicase, each having either an exogenous inverted repeat sequence or a sequence with no repeat. These templates were used to initiate in vitro replication by Q beta replicase in amounts that saturated the enzyme.
V D, Axelrod, E, Brown, C, Priano, D R, Mills   +3 more
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Structure‐Selective Catalytic Alkylation of DNA and RNA

Angewandte Chemie, 2012
A variety of nucleic acids can be catalytically alkylated with rhodium-carbenoids generated from diazo compounds in aqueous buffer through an NH insertion process (see scheme; MES=2-(N-morpholino)ethanesulfonic acid). The method specifically targets unpaired bases such as those present in single strands, turn regions, and overhangs while leaving double-
Tishinov, Kiril, Schmidt, Kristina, Häussinger, Daniel, Gillingham, Dennis   +3 more
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