Results 81 to 90 of about 389,745 (325)

Phylogeny of conserved adenines in linkers of Group-I introns [PDF]

, 2009
We have analyzed the linkers in group-I introns, a characteristic region that is crucial to the folding and splicing process of the folded RNA, in seventy sequences spreading across r-RNA, t-RNA and organelle genes from various organisms including algae,
Chandrasekhar Kesavan, Natarajan Ganesan
core   +1 more source

Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3 [PDF]

, 2004
Colicin E3 is a cytotoxic ribonuclease that specifically cleaves 16S rRNA at the ribosomal A-site to abolish protein synthesis in sensitive Escherichia coli cells.
Bohm, Boon, Bowman, Carr, Cuchillo, Garinot-Schneider, Irie, Kerr, Kleanthous, Kleanthous, Makarov, Mosbahi, Ogawa, Ohno, Ohno, Ohno-Iwashita, Parret, Pommer, Rojas, Scholz, Schultz, Sidikaro, Smarda, Soelaiman, Steege, Tomita, Walker, Walker, Walker, Walker, Wallis   +30 more
core   +2 more sources

Mechanisms and kinetic assays of aminoacyl‐tRNA synthetases

FEBS Letters, EarlyView.
Igor Zivkovic, Morana Dulic, Ita Gruic‐Sovulj   +2 more
wiley   +1 more source

Central Dysmyelination in SSADH‐Deficient Humans and Mice

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives Succinic semialdehyde dehydrogenase deficiency (SSADHD) is an inherited metabolic disorder characterized by an accumulation of γ‐aminobutyric (GABA). In addition to its synaptic role as an inhibitory neurotransmitter, GABA also plays an important role in myelination.
Itay Tokatly Latzer, Henry H. C. Lee, Edward Yang, Cesar Alves, Mariarita Bertoldi, Caitlyn Fung, Spencer V. Steele, Eren Kule, Zijie Jin, Alexander Rotenberg, Jean‐Baptiste Roullet, Phillip L. Pearl   +11 more
wiley   +1 more source

Mechanism of nucleic acid unwinding by SARS-CoV helicase. [PDF]

PLoS ONE, 2012
The non-structural protein 13 (nsp13) of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a helicase that separates double-stranded RNA (dsRNA) or DNA (dsDNA) with a 5' → 3' polarity, using the energy of nucleotide hydrolysis.
Adeyemi O Adedeji, Bruno Marchand, Aartjan J W Te Velthuis, Eric J Snijder, Susan Weiss, Robert L Eoff, Kamalendra Singh, Stefan G Sarafianos   +7 more
doaj   +1 more source

RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding [PDF]

, 2013
For structured RNAs that possess catalytic activity, this activity provides a powerful probe for measuring the progress of folding and the effects of RNA chaperone proteins on the folding rate. The crux of this approach is that only the natively folded RNA is able to perform the catalytic reaction.
Rick Russell, Brant Gracia
openaire   +3 more sources

The Regulation of Trace Metal Elements in Cancer Ferroptosis

Advanced Biology, EarlyView.
The induction of ferroptosis inhibits tumor growth, enhances anticancer efficacy, and overcomes drug resistance. Recent evidence shows nonferrous metal elements play a role in ferroptosis. This review focuses on how trace metals regulate ferroptosis processes like iron accumulation, lipid peroxidation, and antioxidant defense.
Xiaoyan Wang, Yuanyuan Xue, Lei Chang, Xuena Zhu, Wenjun Liu, Tingbo Liang   +5 more
wiley   +1 more source

Rational Design of an Orthogonal Pair of Bimolecular RNase P Ribozymes through Heterologous Assembly of Their Modular Domains

Biology, 2019
The modular structural domains of multidomain RNA enzymes can often be dissected into separate domain RNAs and their noncovalent assembly can often reconstitute active enzymes.
Yuri Nozawa, Megumi Hagihara, Md Sohanur Rahman, Shigeyoshi Matsumura, Yoshiya Ikawa   +4 more
doaj   +1 more source

Influence of Conformation of M. tuberculosis RNase P Protein Subunit on Its Function. [PDF]

PLoS ONE, 2016
RNase P is an essential enzyme that processes 5' end leader sequence of pre-tRNA to generate mature tRNA. The bacterial RNase Ps contain a RNA subunit and one protein subunit, where the RNA subunit contains the catalytic activity.
Alla Singh, Shah Ubaid-ullah, Anup K Ramteke, Janendra K Batra   +3 more
doaj   +1 more source

Investigating the Cellular Effects of GALC Dosing in Enzyme Replacement Therapy for Krabbe Disease Supports the Role of Nanomedicine

Advanced Biology, EarlyView.
A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso, Sara Carpi, Laura Colagiorgio, Miriam De Sarlo, Mariacristina Gagliardi, Marco Cecchini   +5 more
wiley   +1 more source