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Direct Quantification of Protein–Protein Interactions in Living Bacterial Cells
Advanced Science, Volume 12, Issue 19, May 22, 2025.Quantitative measurement of protein–protein interactions in living cells is vital for biotechnology applications. Fluorescence resonance energy transfer (FRET) signals in bacterial cells are often weak, leading to false‐positive. Quantitative FRET measurements are implemented to overcome the weak FRET signal.
Soojung Yi +12 more
wiley +1 more source
Cell division control in Caulobacter crescentus
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2019 Caulobacter crescentus is a free-living Alphaproteobacterium that thrives in oligotrophic environments. This review focuses on the regulatory network used by this bacterium to control the levels of cell division proteins, their organization inside the cell and their activity as a function of the cell cycle. Strikingly, C.
openaire +3 more sources
mBio, 2018 The alphaproteobacterial general stress response is governed by a conserved partner-switching mechanism that is triggered by phosphorylation of the response regulator PhyR.
C. Lori +3 more
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Phosphatase to kinase switch of a critical enzyme contributes to timing of cell differentiation
mBioCell differentiation is an essential biological process that is subject to strict temporal regulation. Caulobacter crescentus undergoes obligate differentiation from a swarmer cell to a stationary, replication-competent stalked cell with each cell cycle.
Trisha N. Chong +3 more
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Isolate and Sub-fractionate Cell Membranes from Caulobacter crescentus
Bio-Protocol, 2012 Cell membranes from Caulobacter can be isolated and separated into inner and outer membranes according their characteristic buoyant densities on a sucrose gradient.
Khatira Anwari
doaj +1 more source
tmRNA Is Required for Correct Timing of DNA Replication in
, 2002 SsrA, or tmRNA, is a small RNA that interacts with selected translating ribosomes to target the nascent polypeptides for degradation. Here we report that SsrA activity is required for normal timing of the G 1-to-S transition in Caulobacter crescentus.
Kenneth C. Keiler +2 more
core
Improved generalized transducing bacteriophage for Caulobacter crescentus [PDF]
Journal of Bacteriology, 1981 Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and ...
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Molecular Systems Biology, 2010 Sensor histidine kinases underlie the regulation of a range of physiological processes in bacterial cells, from chemotaxis to cell division. In the gram‐negative bacterium Caulobacter crescentus, the membrane‐bound histidine kinase, DivJ, is a polar ...
Yihan Lin +2 more
doaj +1 more source
A co-conserved gene pair supports Caulobacter iron homeostasis during chelation stress
Journal of BacteriologySynthetic metal chelators are widely used in industrial, clinical, and agricultural settings, leading to their accumulation in the environment. We measured the growth of Caulobacter crescentus, a soil and aquatic bacterium, in the presence of the ...
Sergio Hernandez-Ortiz +4 more
doaj +1 more source