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Carbohydrate-active enzymes (CAZymes) in the gut microbiome
Nature Reviews Microbiology, 2022The 1013-1014 microorganisms present in the human gut (collectively known as the human gut microbiota) dedicate substantial percentages of their genomes to the degradation and uptake of carbohydrates, indicating the importance of this class of molecules.
Stephen Withers +2 more
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N-glycan breakdown by bacterial CAZymes
Essays in Biochemistry, 2023 Abstract The modification of proteins by N-glycans is ubiquitous to most organisms and they have multiple biological functions, including protecting the adjoining protein from degradation and facilitating communication or adhesion between cells, for example. Microbes have evolved CAZymes to deconstruct different types of N-glycans and
Elizabeth Ficko-Blean, Lucy I Crouch
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The continuing expansion of CAZymes and their families
Current Opinion in Chemical Biology, 2019Carbohydrate-active enzymes (CAZymes) catalyze the assembly and breakdown of glycans and glycoconjugates. Some have been discovered, studied and exploited for numerous applications long ago. For instance, amylase and invertase were isolated in the second half of the 19th century and lysozyme was the first enzyme whose 3-D structure was determined.
Marie-Line Garron, Bernard Henrissat
exaly +5 more sources
Long-Read Metagenomics and CAZyme Discovery
2023Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and ...
Ferrillo, Alessandra +6 more
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CAZyme discovery and design for sweet dreams
Current Opinion in Chemical Biology, 2014Development of synthetic routes to complex carbohydrates and glyco-conjugates is often hampered by the lack of enzymes with requisite properties or specificities. Indeed, assembly or degradation of carbohydrates requires carbohydrate-active enzymes (CAZymes) able to act on a vast range of glycosidic monomers, oligomers or polymers in a regio-specific ...
André, Isabelle +4 more
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Metagenomics and CAZyme Discovery
2017Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems using common culture-dependent techniques restricts access ...
Kunath, Benoit J. +4 more
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Metagenomics and CAZymes in Rumen: A review
Indian Journal of Animal Nutrition, 2019Rumen converts lignocellulsic biomass to high quality food by virtue of the diverse microbiota. The composition of rumen ecosystem is shaped up by various factors including diet, individuality, age, geographic region, postfeeding time etc. Earlier culture based techniques could study rumen microbes partially for the cultivable microbes.
Anju Kala +3 more
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Proteomic Detection of Carbohydrate-Active Enzymes (CAZymes) in Microbial Secretomes
2018Secretomes from microorganisms growing on biomass contain carbohydrate-active enzymes (CAZymes) of potential biotechnological interest. By analyzing such secretomes, we may discover key enzymes involved in degradation processes and potentially infer the mode-of-action of biomass conversion.
Tina R, Tuveng +2 more
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