Results 51 to 60 of about 125,031 (193)

Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles

BMC Genomics, 2005
Background Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor ...
Koopmann Birger, Hettwer Ursula, Venkatesh Balakrishnan, Karlovsky Petr   +3 more
doaj   +1 more source

cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola. [PDF]

BMC Genomics, 2008
AbstractBackgroundThe oomycetePlasmopara viticola(Berk. and Curt.) Berl. and de Toni causes downy mildew in grapevine (Vitis viniferaL.). This pathogen is strictly biotrophic, thus completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We have carried
Polesani M, Desario F, Ferrarini A, Zamboni A, Pezzotti M, Kortekamp A, Polverari A.   +6 more
europepmc   +5 more sources

Development of a simple and powerful method, cDNA AFLP-SSPAG, for cloning of differentially expressed genes [PDF]

, 2010
A novel plant differentially expressed gene cloning technique called cDNA-AFLP-SSPAG was descrybed. Using this method, Differential mRNA expression was found between high and low heterosis groups of maize.
Wu, M
core   +1 more source

The Evolution and Origin of Allotetraploid Aegilops geniculata Revealed by the Homoeolog‐Resolved Genome Assembly

Plant Biotechnology Journal, Volume 24, Issue 3, Page 1866-1884, March 2026.
ABSTRACT Aegilops geniculata Roth is a tetraploid (MgMgUgUg; 2n = 4x = 28) wild relative of wheat and a valuable source of genetic diversity for improving agronomic traits. We present a high‐quality homoeolog‐resolved assembly and annotation of the Ae.
Ural Yunusbaev, Gabriela Romero Campos, Rajendran Sathishraj, Evgenii Liakh, John W. Raupp, Aleksey V. Zimin, Alina Akhunova, Dal‐Hoe Koo, Eduard Akhunov   +8 more
wiley   +1 more source

cDNA-AFLP Combined with Functional Analysis Reveals Novel Genes Involved in the Hypersensitive Response [PDF]

Molecular Plant-Microbe Interactions®, 2006
To identify genes required for the hypersensitive response (HR), we performed expression profiling of tomato plants mounting a synchronized HR, followed by functional analysis of differentially expressed genes. By cDNA-AFLP analysis, the expression profile of tomato plants containing both the Cf-4 resistance gene against Cladosporium fulvum and the ...
Gabriëls, S.H.E.J., Takken, F.L.W., Vossen, J.H., de Jong, C.F., Liu, Q., Turk, S.C.H.J., Wachowski, L.K., Peters, J., Witsenboer, H.M.A., de Wit, P.J.G.M., Joosten, M.H.A.J.   +10 more
openaire   +5 more sources

Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates [PDF]

, 2006
We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses.
Ndung’u, K., Turner, C.M.R., Masiga, D.K., Tweedie, A., Tait, A., Turner, C., Ndungu, K.   +6 more
core   +1 more source

Sorghum Anthracnose (Colletotrichum sublineola) Disease Management and Resistance Breeding Efforts in Eastern Africa

Advances in Agriculture, Volume 2026, Issue 1, 2026.
Sorghum (Sorghum bicolor (L.) Moench) is the fifth most economically important crop in the world, with Eastern Africa recognized as its center of origin and diversity. However, sorghum production faces significant challenges due to biotic and abiotic stress factors. Among the biotic factors, sorghum anthracnose stands out as one of the most devastating
Goytom Berihun Alemayhu, Kumlachew Alemu
wiley   +1 more source

Quantitative comparison of cDNA-AFLP, microarrays, and genechip expression data in Saccharomyces cerevisiae

Genomics, 2003
cDNA-AFLP is a genome-wide expression analysis technology that does not require any prior knowledge of gene sequences. This PCR-based technique combines a high sensitivity with a high specificity, allowing detection of rarely expressed genes and distinguishing between homologous genes.
Reijans, M., Lascaris, R.F., Groeneger, A.O., Wittenberg, A., Wesselink, E., van Oeveren, J., de Wit, A., Boorsma, A., Voetdijk, B., van der Spek, J.C., Grivell, L.A., Simons, G.   +11 more
openaire   +4 more sources

Non PCR-amplified Transcripts and AFLP fragments as reduced representations of the quail genome for 454 Titanium sequencing

BMC Research Notes, 2010
Background SNP (Single Nucleotide Polymorphism) discovery is now routinely performed using high-throughput sequencing of reduced representation libraries. Our objective was to adapt 454 GS FLX based sequencing methodologies in order to obtain the largest
Leterrier Christine, Richard Sabine, Gourichon David, Noirot Céline, Klopp Christophe, Bouchez Olivier, Vignoles Florence, Feve Katia, Leroux Sophie, Beaumont Catherine, Minvielle Francis, Vignal Alain, Pitel Frédérique   +12 more
doaj   +1 more source

Potential Genomic Resources and Their Utilization in Edible Oysters

Aquaculture Research, Volume 2026, Issue 1, 2026.
Oysters have recently been recognized globally as a preferred species, not only for their high‐quality meat and sustainable aquaculture practices but also for their environmental value including carbon sequestration and nitrogen removal in aquaculture ecosystems.
Basanta Pravas Sahu, Mohamed Madhar Fazil Sheik Oli, Subhasmita Panda, Weiwei You, Haigang Qi, Li Li, Vengatesen Thiyagarajan   +6 more
wiley   +1 more source