Results 291 to 300 of about 405,232 (337)

Automation of RNA-Seq Sample Preparation and Miniaturized Parallel Bioreactors Enable High-Throughput Differential Gene Expression Studies. [PDF]

Microorganisms
Blums K, Herzog J, Costa J, Quirico L, Turber J, Weuster-Botz D.   +5 more
europepmc   +1 more source

Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping

Advanced Science, EarlyView.
This study identified two SbALS mutations (A93T and S624N) conferring robust herbicide resistance in sorghum, facilitating efficient weed control. Structural analysis revealed that imazamox resistance is mediated by disrupted herbicide binding. Furthermore, 126 imazamox resistant soybean varieties are screened for sorghum‐soybean intercropping ...
Sanyuan Tang, Jiayang Shi, Xuefeng Li, Mingliang Yang, Chao Li, Dan Zhang, Sen Yang, Cuo Mei, Zuyong Luo, Li Zhang, Wanke Zhang, Chunrui Zhang, Chenbo Zhu, Xiaowei Ma, Ran Xia, Yuhang Chen, Jinsong Zhang, Qingshan Chen, Shouyi Chen, Qi Xie, Feifei Yu   +20 more
wiley   +1 more source

In vivo functional screens reveal <i>KEAP1</i> loss as a driver of chemoresistance in small cell lung cancer. [PDF]

Sci Adv
Brumage L, Best S, Hippe DS, Grunblatt E, Chanana P, Wu F, Lee MC, Ying Z, Ibrahim A, Chung JH, Vigil A, Fatherree J, Beronja S, Paddison P, Sullivan L, Nabet B, MacPherson D.   +16 more
europepmc   +1 more source

Gas Bubbles from Biodegradable Magnesium Implants Convey Mechanical Cues and Promote Immune Cell Stimulation

Advanced Science, EarlyView.
Bubbles in soft tissue are the unavoidable consequence of magnesium implant degradation in patients, yet their biological significance remains unclear. Using advanced imaging and spatial transcriptomics, this study reveals a pronounced inflammatory and mechanical response near bubbles, contrasting with pro‐regenerative events closer to the implant ...
Heithem Ben Amara, Jincy Philip, Omar Omar, Peter Thomsen   +3 more
wiley   +1 more source

The influence of the geroprotective cytokine on the transcriptome of young and senescent mesenchymal stem cells. [PDF]

BMC Res Notes
Kondratyeva LG, Matveeva DK, Ezdakova MI, Utkina MV, Ratushnyy AY.   +4 more
europepmc   +1 more source

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Normalizing cDNA Libraries

Current Protocols in Molecular Biology, 2010
AbstractThe characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare ...
Sergey Lukyanov, Dmitry A. Shagin, Irina A. Shagina, Ilya V. Kelmanson, Ekaterina A. Bogdanova, Ekaterina V. Barsova   +5 more
openaire   +3 more sources

Normalization of cDNA Libraries [PDF]

, 2007
In a cellular transcriptome, the number of mRNA copies per gene may differ by several orders. In cDNA libraries, performed from mRNA, these proportions are the same. Normalization methods allow us to equalize numbers of gene’s copies in the library. Normalized cDNA libraries are used to discover new genes transcribed at relatively low levels or for ...
Dmitry A. Shagin, Ekaterina A. Bogdanova, Pavel A. Zhulidov, Alex S. Shcheglov   +3 more
openaire   +1 more source