Results 41 to 50 of about 4,356 (168)

Immunoprecipitation of CDT Complexes Containing Deletion Mutants of CdtA

, 2013
(A) The complexes (10 μg each) were immunoprecipitated with 10 μg anti-myc antibody (myc tag was fused to C-terminus of CdtC) in 150 mM NaCl, 20 mM HEPES (pH 7.5), 0.1% Nonidet P40 substitute, followed by exposure to protein G Sepharose beads, and ...
Dragana Nesic (60577), C. Erec Stebbins (60578)   +1 more
core   +1 more source

Laser induced breakdown spectroscopy in water

Journal of Applied Sciences and Environmental Management, 2005
Preliminary experimental results are reported on the temporal characteristics of laser spark emission spectra. Sparks were generated in water by the focused beam of a Q-switched Nd:YAG laser Na and Cu aqueous solutions exhibited fluorescence signal on ...
S Boudjemai, T Gasmi, R Boushaki, R Kasbadji, F Medjahed   +4 more
doaj   +1 more source

A Single Amino Acid Substitution in CspA Increases Germination Sensitivity and Broadens Bile Salt Germinant Specificity in Clostridioides difficile Spores

Environmental Microbiology, Volume 28, Issue 6, June 2026.
In reservoir‐associated RT033 strains, we observe a natural rewiring of spore germination that lowers the activation threshold and broadens bile‐salt specificity, enabling germination at low germinant levels and in response to typically inhibitory bile‐salts common in animal hosts.
Isabel Roseiro, Alexandra Nunes, Diogo Martins, Frederico Alves, Søren Persson, Adriano O. Henriques, Mónica Oleastro, Mónica Serrano   +7 more
wiley   +1 more source

Shared Decision Making: Aids and Promotion in Clinical Practice Guidelines for Type 2 Diabetes Treatment

Journal of Evaluation in Clinical Practice, Volume 32, Issue 4, June 2026.
ABSTRACT Introduction Type 2 diabetes (T2D) is a major global health issue due to its rising prevalence and significant burden in terms of morbidity, mortality, and economic impact. Although the availability of Clinical Practice Guidelines (CPGs) aimed at optimising T2D management, the complexity of therapeutic options and the necessity of tailoring ...
Yves Marie Vincent, Merwan Kasbi, Reda Saouli, Blanchard Clara, Rémy Boussageon   +4 more
wiley   +1 more source

Salicylic Acid Delays Grape Softening by Regulating Cell Wall, Cuticular Wax, and Phenylpropanoid Metabolism

Food Frontiers, Volume 7, Issue 3, May 2026.
This study investigated the effects of salicylic acid (SA) application on the softening of grapes (Vitis vinifera L. ‘Red Globe’) during cold storage. The results demonstrated that SA treatment effectively delayed softening by regulating cell wall, cuticular wax, and phenylpropanoid metabolism.
Guihua Zeng, Haiyan Liu, Yuyun He, Jianming Niu, Ping Wang, Qinming Sun, Feifei Gao   +6 more
wiley   +1 more source

History of the Electrical Characterization and Test Platform Development at Microelectronics and Nanotechnology Division of CDTA, Algeria

Algerian Journal of Signals and Systems
The sixties (60s) and seventies (70s) of the last century have seen the takeoff of microelectronics. Since that, several countries, including Algeria, have adopted planned strategic programs to develop their domestic electronics.
Boualem Djezzar
doaj   +1 more source

Molecular and conventional methods for detection of Campylobacter spp. in retail poultry cuts, offal and chicken products [PDF]

Benha Veterinary Medical Journal, 2019
The goal of this study is to determine the prevalence of Campylobacter spp. in some poultry products by using both conventional and molecular methods. Two hundred chicken product samples were collected from various supermarkets at Menofia government (25g
Amani Salem, Nahla Abou El Roos, Yahya Nassar   +2 more
doaj   +1 more source

Microbiome‐urothelium crosstalk in bladder cancer: From dysbiosis to clinical translation

iMetaOmics, Volume 3, Issue 1, March 2026.
This review elucidates the critical crosstalk between the urobiome and bladder cancer (BCa), mapping the landscape from ecological dysbiosis to clinical translation. We synthesize emerging evidence on microbial signatures that distinguish BCa patients, exploring key carcinogenic mechanisms including chronic inflammation, genotoxicity, and the gut ...
Haoxiang Xu, Zhibiao Li, Lin Yang, Jun Zheng, Weijia Li, Rulin Liao, Wenxue Huang, Boyuan Sun, Zhuohang Li, Cunzhen Ma, Xunguo Yang, Zongwei Wang, Jie Zhao, Bisheng Cheng, Peng Wu   +14 more
wiley   +1 more source

Cytolethal distending toxin (CDT)-negative Campylobacter jejuni strains and anti-CDT neutralising antibodies induced during human infection but not chicken colonisation [PDF]

, 2005
The cytolethal distending toxin (CDT) of Campylobacter jejuni was detectable, using an in vitro assay, in most but not all of 24 strains tested. The reason for the absence of toxin activity in these naturally occurring CDT-negative C.
Ridley, A, Wassenaar, TM, Ahmed, IH, Cawthraw, SA, AbuOun, M, Manning, G, Newell, DG   +6 more
core  

Effect of recombinant CdtA proteins and CDT holotoxins on subunits binding.

, 2013
A. Bound protein were detected with His Tag monoclonal antibody (1:500 dilution) and TRITC-Labeled Goat Anti-Mouse IgG second antibody (1∶180 dilution). Samples were co-stained with DAPI dye to visualized the membrane of CHO cells.
Ying Sun (23237), Yan Xu (14594), Cheng Ding (34937), Xiao-qian Wang (417736), Mi-fang Yang (5654959), Lu Li (14069), Jun-lan Duan (417735)   +6 more
core   +1 more source