Results 111 to 120 of about 4,735,099 (379)

Protocol for establishing primary murine colonic fibroblast culture with induction of loxP/loxP deletion using transducible TAT-Cre recombinase

STAR Protocols
Summary: Conditional mutagenesis using site-specific recombinases is widely used in transgenic mouse models; however, its efficiency in vitro is limited by poor tamoxifen activity.
Christina McAninch, Allison Hahn, Steven McAninch, Irina V. Pinchuk, Marina Chulkina   +4 more
doaj   +1 more source

Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays [PDF]

, 2018
This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture ...
Daza Navarro, María Paula, Huertas Sánchez, Gloria, Maldonado-Jacobi, Andrés, Martín Rubio, María Esther, Olmo Fernández, Alberto, Pérez García, Pablo, Serrano, Juan A., Yúfera García, Alberto   +7 more
core   +1 more source

Cell-based assays in high-throughput mode (HTS)

, 2016
Typically, novel compounds are screened by the millions, a process known as high-throughput screening (HTS). HTS allows for the screening of millions of potential drugs in a relatively short period of time.
Kalyani Rajalingham
semanticscholar   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy including senscence, necrosis, and autophagy, but not apoptosis [PDF]

, 2015
In comparison to more differentiated cells, prostate cancer stem-like cells are radioresistant, which could explain radio-recurrent prostate cancer. Improvement of radiotherapeutic efficacy may therefore require combination therapy.
Boyle, Ross W., Bryden, Francesca, Frame, Fiona M., Giuntini, Francesca, Maitland, Norman J., Mann, Vincent M., Savoie, Huguette, Simms, Matthew S.   +7 more
core   +1 more source

On-chip ultrasonic sample preparation for cell based assays†

, 2015
We demonstrate an acoustophoresis method for size-based separation, isolation, up-concentration and trapping of cells that can be used for on-chip sample preparation combined with high resolution imaging for cell-based assays.
I. Iranmanesh, H. Ramachandraiah, A. Russom, M. Wiklund   +3 more
semanticscholar   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

A single extracellular amino acid in Free Fatty Acid Receptor 2 defines antagonist species selectivity and G protein selection bias [PDF]

, 2017
Free Fatty Acid Receptor 2 is a GPCR activated by short chain fatty acids produced in high levels in the lower gut by microbial fermentation of non-digestible carbohydrates.
Aoki, Junken, Bolognini, Daniele, Hansen, Anders Højgaard, Hudson, Brian D., Inoue, Asuka, Kawakami, Kouki, Kishi, Takayuki, Milligan, Graeme, Sergeev, Eugenia, Ulven, Trond   +9 more
core   +3 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion

STAR Protocols
Summary: Site-specific fluorescent labeling of HIV-1 proteins enables direct visualization of their dynamics and trafficking. Here, we present a protocol to label HIV-1 envelope (Env) glycoproteins on virions using genetic code expansion and click ...
Wang Xu, Narendra Kumar Gonepudi, Maolin Lu   +2 more
doaj   +1 more source