Results 111 to 120 of about 4,481,323 (329)

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

STAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj  

A chemical susceptibility profile of the Plasmodium falciparum transmission stages by complementary cell-based gametocyte assays.

Journal of Antimicrobial Chemotherapy, 2016
OBJECTIVES As most available antimalarial drugs are ineffective against the Plasmodium falciparum transmission stages, new drugs against the parasite's gametocytes are urgently needed to combat malaria globally. The unique biology of gametocytes requires
S. D’Alessandro, G. Camarda, Y. Corbett, Giulia Siciliano, S. Parapini, L. Cevenini, E. Michelini, A. Roda, D. Leroy, D. Taramelli, P. Alano   +10 more
semanticscholar   +1 more source

SINGLE STEP FLUORESCENT CASPASE 3 ACTIVITY ASSAY: QUANTIFYING THE INHIBITION OF CELL PERMEABLE COMPOUNDS IN A CELL-BASED SYSTEM [PDF]

, 2001
Jean Humpal-Winter, Andrew L. Niles, Martha A. O′Brien, Bob Bulleit   +3 more
openalex   +2 more sources

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

STAR Protocols, 2021
Summary: In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro.
Caroline Perner, Caroline L. Sokol
doaj  

A METHOD OF ASSAYING ADRENAL CORTICAL HORMONES BASED ON A DECREASE IN THE CIRCULATING EOSINOPHIL CELLS OF ADRENALECTOMIZED MICE¹ [PDF]

, 1951
R. S. Speirs, Roland K. Meyer
openalex   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

STAR Protocols, 2020
Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response.
Wan Hon Koh, Romaniya Zayats, Paul Lopez, Thomas T. Murooka   +3 more
doaj  

A protocol for imaging microvilli biogenesis on the surface of cultured porcine kidney epithelial cell monolayers

STAR Protocols, 2021
Summary: A key facet of epithelial differentiation is the assembly of actin-based protrusions known as microvilli, which amplify apical membrane surface area for various cell functions. To probe mechanisms of microvillus assembly, we developed a protocol
Isabella M. Gaeta, Leslie M. Meenderink, Matthew J. Tyska   +2 more
doaj  

A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity. [PDF]

, 1990
George D. Yancopoulos, Garry P. Nolan, Roberta R. Pollock, Susan E. Prockop, Sing‐Chung Li, L A Herzenberg, Frederick W. Alt   +6 more
openalex   +1 more source