Results 131 to 140 of about 4,481,323 (329)

Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

STAR Protocols
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering.
Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao   +3 more
doaj  

Enzyme-based Antigen Localization and Quantitation in Cell and Tissue Samples (Midwestern Assay) [PDF]

, 1997
Kevin A. Roth, Jennifer W. Brenner, Lee A. Selznick, Murat Gökden, Robin G. Lorenz   +4 more
openalex   +1 more source

SpotNet - Learned iterations for cell detection in image-based immunoassays

, 2019
Accurate cell detection and counting in the image-based ELISpot and FluoroSpot immunoassays is a challenging task. Recently proposed methodology matches human accuracy by leveraging knowledge of the underlying physical process of these assays and using ...
Jaldén, Joakim, Pla, Pol del Aguila, Saxena, Vidit   +2 more
core   +1 more source

Subpar reporting of pre‐analytical variables in RNA‐focused blood plasma studies

Molecular Oncology, EarlyView.
Pre‐analytical variables strongly influence the analysis of extracellular RNA (cell‐free RNA; exRNA) derived from blood plasma. Their reporting is essential to allow interpretation and replication of results. By evaluating 200 exRNA studies, we pinpoint a lack of reporting pre‐analytical variables associated with blood collection, plasma preparation ...
Céleste Van Der Schueren, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Laudonia Lidia Dipalo, Hetty Hilde Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, Anneleen Decock   +16 more
wiley   +1 more source

Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures

STAR Protocols
Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures.
Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber   +3 more
doaj  

Identification of functional and diverse circulating cancer‐associated fibroblasts in metastatic castration‐naïve prostate cancer patients

Molecular Oncology, EarlyView.
Cancer‐associated fibroblasts (CAFs) promote cancer growth, invasion (metastasis), and drug resistance. Here, we identified functional and diverse circulating CAFs (cCAFs) in patients with metastatic prostate cancer (mPCa). cCAFs were found in higher numbers and were functional and diverse in mPCa patients versus healthy individuals, suggesting their ...
Richell Booijink, Leon W. M. M. Terstappen, Eshwari Dathathri, Khrystany Isebia, Jaco Kraan, John Martens, Ruchi Bansal   +6 more
wiley   +1 more source

Mouse N2a Neuroblastoma Assay: Uncertainties and Comparison with Alternative Cell-Based Assays for Ciguatoxin Detection. [PDF]

Mar Drugs, 2023
Raposo-Garcia S, Cao A, Costas C, Louzao MC, Vilariño N, Vale C, Botana LM.   +6 more
europepmc   +1 more source

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source

Fixed cell-based assays for autoantibody detection in myasthenia gravis: a diagnostic breakthrough. [PDF]

Lancet Reg Health West Pac, 2023
Budhram A.
europepmc   +1 more source

Covalent Binding of the Benzamide RH-4032 to Tubulin in Suspension-Cultured Tobacco Cells and Its Application in a Cell-Based Competitive-Binding Assay [PDF]

, 2000
David H. Young, Veronica T. Lewandowski
openalex   +1 more source