Results 181 to 190 of about 4,481,323 (329)

Protocol to assess bioenergetics and mitochondrial fuel usage in murine autoreactive immunocytes using the Seahorse Extracellular Flux Analyzer

STAR Protocols
Summary: Efficient metabolism, or the means by which cells produce energy resources, is critical for proper effector function. Here, we present a protocol for examining the bioenergetics and mitochondrial fuel utilization of primary murine autoreactive ...
Colleen L. Mayberry, John J. Wilson, Britney Sison, Chih-Hao Chang   +3 more
doaj  

Prozone phenomenon in pretransplant testing: An interesting conundrum involving solid-phase and cell-based assays. [PDF]

Asian J Transfus Sci, 2022
Singh P, Tiwari AK, Mishra VC, Deshpande TV, Dorwal P, Bhardwaj AK, Kumari S, Raina V.   +7 more
europepmc   +1 more source

Addressing persistent challenges in digital image analysis of cancer tissue: resources developed from a hackathon

Molecular Oncology, EarlyView.
Large multidimensional digital images of cancer tissue are becoming prolific, but many challenges exist to automatically extract relevant information from them using computational tools. We describe publicly available resources that have been developed jointly by expert and non‐expert computational biologists working together during a virtual hackathon
Sandhya Prabhakaran, Clarence Yapp, Gregory J. Baker, Johanna Beyer, Young Hwan Chang, Allison L. Creason, Robert Krueger, Jeremy Muhlich, Nathan Heath Patterson, Kevin Sidak, Damir Sudar, Adam J. Taylor, Luke Ternes, Jakob Troidl, Xie Yubin, Artem Sokolov, Darren R. Tyson   +16 more
wiley   +1 more source

Protocol to calculate the synergy of drug combinations in organoids and primary cells from murine tumors

STAR Protocols
Summary: Evaluating the synergy of drug combinations is crucial in advancing treatment regimens. Here, we present a protocol to establish primary cells and organoids from murine tumors and calculate drug synergy.
David Eisenbarth, Bomin Ku, Dae-Sik Lim
doaj  

Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays. [PDF]

ALTEX, 2022
House JS, Grimm FA, Klaren WD, Dalzell A, Kuchi S, Zhang SD, Lenz K, Boogaard PJ, Ketelslegers HB, Gant TW, Rusyn I, Wright FA.   +11 more
europepmc   +1 more source

Discovery of Novel Ansamycins Possessing Potent Inhibitory Activity in a Cell-based Oncostatin M Signalling Assay.

, 2000
Paul Stead, Shazia Latif, Andrew P. Blackaby, Philip J. Sidebottom, Angela M. Deakin, NICHOLAS L. TAYLOR, Paul Life, John R. Spaull, Fiona Burrell, Rosie Jones, JANE LEWIS, Ian Davidson, Thomas Mander   +12 more
openalex   +2 more sources

New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population [PDF]

, 2003
Inge Jedema, Nicole M. van der Werff, Renée M. Y. Barge, Roel Willemze, J.H. Frederik Falkenburg   +4 more
openalex   +1 more source

Detection rate for ESR1 mutations is higher in circulating‐tumor‐cell‐derived genomic DNA than in paired plasma cell‐free DNA samples as revealed by ddPCR

Molecular Oncology, EarlyView.
Analysis of ESR1 mutations in plasma cell‐free DNA (cfDNA) is highly important for the selection of treatment in patients with breast cancer. Using multiplex‐ddPCR and identical blood draws, we investigated whether circulating tumor cells (CTCs) and cfDNA provide similar or complementary information for ESR1 mutations.
Stavroula Smilkou, Aliki Ntzifa, Victoria Tserpeli, Ioanna Balgkouranidou, Alkistis Papatheodoridi, Evangelia Razis, Helena Linardou, Christos Papadimitriou, Amanda Psyrri, Flora Zagouri, Stylianos Kakolyris, Evi Lianidou   +11 more
wiley   +1 more source

Protocol for real-time measurement of mitochondrial bioenergetics in 3D-cultured brain tumor stem cells using the Resipher system

STAR Protocols
Summary: Aberrant mitochondrial function can lead to severe human diseases, including neurodegenerative diseases and cancer. Here, we describe a cell-based protocol for measuring different mitochondrial respiratory parameters using the high-resolution ...
Cloé Tessier, Maxime Toujas, Antonio C. Pagano Zottola, Andreas Bikfalvi, Thomas Mathivet, Thomas Daubon, Lucie Brisson, Audrey Burban, Ahmad Sharanek   +8 more
doaj  

Microtiter Cell-Based Assay for Detection of Agents that Alter Cellular Levels of Her2 and EGFR [PDF]

, 2003
Henri Huezo, Maria Vilenchik, Neal Rosen, Gabriela Chiosis   +3 more
openalex   +1 more source