Results 101 to 110 of about 1,006,135 (293)

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

Nature Communications, 2018
Cell-free protein synthesis allows for producing proteins without the need of a host organism, thus sparing the researcher experimental hassle. Here, the authors developed a cell-free synthesis method that enables incorporating non-standard amino acids ...
Rey W. Martin, Benjamin J. Des Soye, Yong-Chan Kwon, Jennifer Kay, Roderick G. Davis, Paul M. Thomas, Natalia I. Majewska, Cindy X. Chen, Ryan D. Marcum, Mary Grace Weiss, Ashleigh E. Stoddart, Miriam Amiram, Arnaz K. Ranji Charna, Jaymin R. Patel, Farren J. Isaacs, Neil L. Kelleher, Seok Hoon Hong, Michael C. Jewett   +17 more
doaj   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Cell-free Protein Synthesis Extract Preparation Methods [PDF]

, 2013
My ORCA project proposed to test and optimize various low capital cost cell lysis methods, while leaving the internal cell machinery intact and viable for cell-free protein synthesis research. Our cells of interest were a strain of the bacterium E. coli,
Holland, Troy, Bundy, Dr. Brad
core  

Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

Synthetic and Systems Biotechnology
Chinese hamster ovary (CHO) cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications.
Jeffrey L. Schloßhauer, Lena Tholen, Alexander Körner, Stefan Kubick, Sofia Chatzopoulou, Anja Hönow, Anne Zemella   +6 more
doaj   +1 more source

Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System

Methods and Protocols, 2019
Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics.
Wei Gao, Ning Bu, Yuan Lu
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

Analysis of leukocyte membrane protein interactions using protein microarrays [PDF]

, 2005
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work
Foster-Cuevas, M, Letarte, Michelle, Deborah Hatherley, Foster-Cuevas, Mildred, Hatherley, D, Barclay, AN, Michelle Letarte, Saunders, Nigel, A Neil Barclay, Saunders, NJ, Hatherley, Deborah, Letarte, M, Barclay, Neil, Mildred Foster-Cuevas, Nigel J Saunders, Saunders, Nigel J, Voulgaraki, D, Despina Voulgaraki, Voulgaraki, Despina   +18 more
core   +2 more sources

The therapeutic opportunities and pitfalls of using iron ion chelators to treat neurodegenerative diseases

FEBS Letters, EarlyView.
Although too much iron in the brain promotes neurodegeneration, iron ion chelators have had mixed effects in clinical trials. This review explains why; some chelators do not render the iron redox‐inactive (e.g., L1) whereas others do (e.g., desferrioxamine).
Barry Halliwell
wiley   +1 more source

Cell-free protein synthesis for nonribosomal peptide synthetic biology

Frontiers in Natural Products
Peptide natural products have a wide range of useful applications as pesticides, veterinary agents, pharmaceuticals, and bioproducts. To discover new natural products, manipulate them for analog generation, and to harness the potential of these bioactive
Tien T. Sword, Ghaeath S. K. Abbas, Constance B. Bailey   +2 more
doaj   +1 more source