Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy [PDF]
Cells, 2021 Propagation of paternal sperm-contributed mitochondrial genes, resulting in heteroplasmy, is seldom observed in mammals due to post-fertilization degradation of sperm mitochondria, referred to as sperm mitophagy.
Won-Hee Song +6 more
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Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability.
Jiaojiao Li +5 more
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O2-Tuned Protein Synthesis Machinery in Escherichia coli-Based Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Involved in most aerobic biochemical processes, oxygen affects cellular functions, and organism behaviors. Protein synthesis, as the underlying biological process, is unavoidably affected by the regulation of oxygen delivery and utilization.
Xiaomei Lin +5 more
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A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha [PDF]
Biology, 2022 Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim of the
Nawal Abd El-Baky +5 more
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A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins [PDF]
BioengineeringCell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation ...
Jeffrey L. Schloßhauer +3 more
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Translation initiation consistency between in vivo and in vitro bacterial protein expression systems
Frontiers in Bioengineering and Biotechnology, 2023 Strict on-demand control of protein synthesis is a crucial aspect of synthetic biology. The 5′-terminal untranslated region (5′-UTR) is an essential bacterial genetic element that can be designed for the regulation of translation initiation.
Jiaojiao Li +10 more
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Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals [PDF]
The Ukrainian Biochemical Journal, 2020 The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was ...
A. K. Gulevskyy +2 more
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An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation
eLife, 2022 The ring-like ATPase complexes in the AAA+ family perform diverse cellular functions that require coordination between the conformational transitions of their individual ATPase subunits (Erzberger and Berger, 2006; Puchades et al., 2020).
Rui Fang +3 more
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Frontiers in Bioengineering and Biotechnology, 2022 Kinetic modeling has relied on using a tedious number of mathematical equations to describe molecular kinetics in interacting reactions. The long list of differential equations with associated abstract variables and parameters inevitably hinders readers’
Yijie Deng +5 more
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Cell-Free PURE System: Evolution and Achievements
BioDesign Research, 2022 The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an in vitro open environment without a complete living cell.
Yi Cui, Xinjie Chen, Ze Wang, Yuan Lu
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