OnePot PURE Cell-Free System. [PDF]
Journal of Visualized Experiments, 2021 The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited.
Laura Grasemann +3 more
semanticscholar +3 more sources
Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy [PDF]
Cells, 2021 Propagation of paternal sperm-contributed mitochondrial genes, resulting in heteroplasmy, is seldom observed in mammals due to post-fertilization degradation of sperm mitochondria, referred to as sperm mitophagy.
Won-Hee Song +6 more
doaj +2 more sources
Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability.
Jiaojiao Li +5 more
doaj +2 more sources
A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds. [PDF]
Metab Eng Commun, 2020 The need for sustainable, low-cost production of bioenergy and commodity chemicals is increasing. Unfortunately, the engineering potential of whole-cell catalysts to address this need can be hampered by cellular toxicity.
Kay JE, Jewett MC.
europepmc +2 more sources
O2-Tuned Protein Synthesis Machinery in Escherichia coli-Based Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Involved in most aerobic biochemical processes, oxygen affects cellular functions, and organism behaviors. Protein synthesis, as the underlying biological process, is unavoidably affected by the regulation of oxygen delivery and utilization.
Xiaomei Lin +5 more
doaj +2 more sources
Production of G protein-coupled receptors in an insect-based cell-free system. [PDF]
Biotechnol Bioeng, 2017 The biochemical analysis of human cell membrane proteins remains a challenging task due to the difficulties in producing sufficient quantities of functional protein. G protein‐coupled receptors (GPCRs) represent a main class of membrane proteins and drug
Sonnabend A +5 more
europepmc +2 more sources
Amyloid β directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system. [PDF]
Inflamm Regen, 2018 Alzheimer’s disease is a neurodegenerative disease characterized by the interstitial deposition of amyloid β (Aβ) plaque, which is thought to be related to chronic neuroinflammation. Aβ is known to make fibrils via oligomers from monomers.
Nakanishi A +10 more
europepmc +2 more sources
High-yield production of "difficult-to-express" proteins in a continuous exchange cell-free system based on CHO cell lysates. [PDF]
Sci Rep, 2017 Cell-free protein synthesis (CFPS) represents a promising technology for efficient protein production targeting especially so called “difficult-to-express” proteins whose synthesis is challenging in conventional in vivo protein production platforms ...
Thoring L +4 more
europepmc +2 more sources
A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha [PDF]
Biology, 2022 Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim of the
Nawal Abd El-Baky +5 more
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Protein synthesis using a reconstituted cell-free system. [PDF]
Curr Protoc Mol Biol, 2014 Most cell‐free protein‐synthesis systems are based on cell extracts, which often contain undesirable activities. Reconstituted systems, by contrast, are composed of a defined number of purified and recombinant components with minimal nuclease and ...
Tuckey C, Asahara H, Zhou Y, Chong S.
europepmc +2 more sources