Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy [PDF]
Cells, 2021 Propagation of paternal sperm-contributed mitochondrial genes, resulting in heteroplasmy, is seldom observed in mammals due to post-fertilization degradation of sperm mitochondria, referred to as sperm mitophagy.
Won-Hee Song +6 more
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Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability.
Jiaojiao Li +5 more
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O2-Tuned Protein Synthesis Machinery in Escherichia coli-Based Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Involved in most aerobic biochemical processes, oxygen affects cellular functions, and organism behaviors. Protein synthesis, as the underlying biological process, is unavoidably affected by the regulation of oxygen delivery and utilization.
Xiaomei Lin +5 more
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A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha [PDF]
Biology, 2022 Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim of the
Nawal Abd El-Baky +5 more
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A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins [PDF]
BioengineeringCell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation ...
Jeffrey L. Schloßhauer +3 more
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Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses [PDF]
Scientific Reports, 2017 Membrane proteins are key elements in cell-mediated processes. In particular, G protein-coupled receptors (GPCRs) have attracted increasing interest since they affect cellular signaling.
Anne Zemella +5 more
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Translation initiation consistency between in vivo and in vitro bacterial protein expression systems
Frontiers in Bioengineering and Biotechnology, 2023 Strict on-demand control of protein synthesis is a crucial aspect of synthetic biology. The 5′-terminal untranslated region (5′-UTR) is an essential bacterial genetic element that can be designed for the regulation of translation initiation.
Jiaojiao Li +10 more
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Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals [PDF]
The Ukrainian Biochemical Journal, 2020 The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was ...
A. K. Gulevskyy +2 more
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OnePot PURE Cell-Free System [PDF]
Journal of Visualized Experiments, 2021 The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In comparison, a home-made approach can be customized based on user needs.
Grasemann, Laura +3 more
openaire +2 more sources
An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation
eLife, 2022 The ring-like ATPase complexes in the AAA+ family perform diverse cellular functions that require coordination between the conformational transitions of their individual ATPase subunits (Erzberger and Berger, 2006; Puchades et al., 2020).
Rui Fang +3 more
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