Results 1 to 10 of about 1,006,135 (293)
CO2-elevated cell-free protein synthesis [PDF]
Synthetic and Systems Biotechnology, 2022 Gases are the vital nutrition of all organisms as the precursor of metabolism pathways. As a potential biological process, protein synthesis is inevitably regulated by gas transport and utilization.
Xiaomei Lin +7 more
doaj +6 more sources
A User’s Guide to Cell-Free Protein Synthesis [PDF]
Methods and Protocols, 2019 Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more.
Nicole E. Gregorio +2 more
doaj +5 more sources
Cell-free protein synthesis for proteomics [PDF]
Briefings in Functional Genomics & Proteomics, 2004 The use of cell-free expression systems as an alternative to cell-based methods for protein production is greatly facilitating studies of protein functions. Recent improvements to cell-free systems, and the development of cell-free protein display and microarray technologies, have led to cell-free protein synthesis becoming a powerful tool for large ...
Mingyue He, He Mingyue
exaly +3 more sources
Advancing synthetic biology through cell-free protein synthesis [PDF]
Computational and Structural Biotechnology Journal, 2023 The rapid development of synthetic biology has enabled the production of compounds with revolutionary improvements in biotechnology. DNA manipulation tools have expedited the engineering of cellular systems for this purpose.
Ke Yue, Junyu Chen, Yingqiu Li, Lei Kai
doaj +2 more sources
IRES-mediated Pichia pastoris cell-free protein synthesis [PDF]
Bioresources and Bioprocessing, 2023 Cell-free protein synthesis (CFPS) system is an ideal platform for fast and convenient protein research and has been used for macromolecular assembly, unnatural amino acid embedding, glycoprotein production, and more.
Yanan Wang +3 more
doaj +2 more sources
Supramolecular protein assembly in cell-free protein synthesis system [PDF]
Bioresources and Bioprocessing, 2022 Protein-based biomaterials have the characteristics of stability and biocompatibility. Based on these advantages, various bionic materials have been manufactured and used in different fields.
Zhixia Li +6 more
doaj +2 more sources
Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways [PDF]
Microorganisms, 2022 As a fast and reliable technology with applications in diverse biological studies, cell-free protein synthesis has become popular in recent decades. The cell-free protein synthesis system can be considered a complex chemical reaction system that is also ...
Anwesha Purkayastha +2 more
doaj +2 more sources
Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery [PDF]
BioTech, 2021 We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus ...
Marina Snapyan +5 more
doaj +2 more sources
“Cell-Free Synthetic Biology”: Synthetic Biology Meets Cell-Free Protein Synthesis [PDF]
Methods and Protocols, 2019 Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [...]
Seok Hoon Hong
doaj +3 more sources
Applications of cell free protein synthesis in protein design. [PDF]
Protein SciAbstractIn protein design, the ultimate test of success is that the designs function as desired. Here, we discuss the utility of cell free protein synthesis (CFPS) as a rapid, convenient and versatile method to screen for activity. We champion the use of CFPS in screening potential designs.
Thornton EL +5 more
europepmc +3 more sources