Results 21 to 30 of about 1,006,135 (293)

Optimising protein synthesis in cell‐free systems, a review

Engineering Biology, 2021
Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology.
Angelo C. Batista, Paul Soudier, Manish Kushwaha, Jean‐Loup Faulon   +3 more
doaj   +1 more source

A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells. [PDF]

PLoS ONE, 2014
In this study, we present a novel technique for the synthesis of complex prokaryotic and eukaryotic proteins by using a continuous-exchange cell-free (CECF) protein synthesis system based on extracts from cultured insect cells.
Marlitt Stech, Robert B Quast, Rita Sachse, Corina Schulze, Doreen A Wüstenhagen, Stefan Kubick   +5 more
doaj   +1 more source

Glycosylation-on-a-Chip: A Flow-Based Microfluidic System for Cell-Free Glycoprotein Biosynthesis

Frontiers in Molecular Biosciences, 2021
In recent years, cell-free synthetic glycobiology technologies have emerged that enable production and remodeling of glycoproteins outside the confines of the cell.
Alicia K. Aquino, Zachary A. Manzer, Susan Daniel, Matthew P. DeLisa, Matthew P. DeLisa   +4 more
doaj   +1 more source

Artificial environments for the co-translational stabilization of cell-free expressed proteins [PDF]

, 2013
An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of ...
Volker Dötsch, Dötsch, Volker, Frank Bernhard, Kai, Lei, Kaldenhoff, Ralf, Lei, Kai, Lei Kai, Ralf Kaldenhoff, Bernhard, Frank   +8 more
core   +1 more source

A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties [PDF]

, 2022
S.57-77Fluorescent labeling of de novo synthesized proteins is in particular a valuable tool for functional and structural studies of membrane proteins.
Thoring, L., Richter, T., Kubick, S., Zemella, A.   +3 more
core   +1 more source

Biochemical aspects of bacterial strategies for handling the incomplete translation processes

Frontiers in Microbiology, 2014
During protein synthesis in cells, translating ribosomes may encounter abnormal situations that lead to retention of immature peptidyl-tRNA on the ribosome due to failure of suitable termination processes.
Yoshihiro eShimizu
doaj   +1 more source

Cell-free protein synthesis of membrane (1,3)-beta-D-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs [PDF]

, 2013
A membrane-embedded curdlan synthase (CrdS) from Agrobacterium is believed to catalyse a repetitive addition of glucosyl residues from UDP-glucose to produce the (1,3)-β-d-glucan (curdlan) polymer.
Mertens, Haydyn D.T., Garajová, Soňa, Hrmova, Maria, Garajova, S., Hrmova, M., Mertens, H., Waters, Shane, Amalraj, Amritha, Nagarajan, Yagnesh, Nagarajan, Y., Waters, S., Periasamy, Agalya, Shadiac, N., Periasamy, A., Shadiac, Nadim, Amalraj, A.   +15 more
core   +1 more source

Energy regeneration systems in cell-free protein synthesis in vitro [PDF]

, 2022
S.67-76Cell-free protein synthesis is a rapidly evolving technology that presents an alternative to conventional recombinant protein production. The technology utilizes cell lysates for ex situ protein synthesis.
Schwedhelm, Ivo, Monaghan, Michael, Thein, Marcus, Hansmann, Jan, Kahlig, Alexander   +4 more
core  

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes [PDF]

, 2010
The continuous progress in the structural and functional characterization of aquaporins increasingly attracts attention to study their roles in certain mammalian diseases.
Volker Dötsch, Lian, Jiazhang, Kaldenhoff Ralf, Dötsch, Volker, Frank Bernhard, Xiangcheng Zhu, Cen, Peilin, Dötsch Volker, Lei Kai, Zhinan Xu, Xu, Zhinan, Peilin Cen, Kai Lei, Kai, Lei, Cen Peilin, Kaldenhoff, Ralf, Xu Zhinan, Zhu Xiangcheng, Ralf Kaldenhoff, Lian Jiazhang, Jiazhang Lian, Bernhard, Frank, Zhu, Xiangcheng, Bernhard Frank   +23 more
core   +1 more source

The Effect of the Protein Synthesis Entropy Reduction on the Cell Size Regulation and Division Size of Unicellular Organisms

Entropy, 2022
The underlying mechanism determining the size of a particular cell is one of the fundamental unknowns in cell biology. Here, using a new approach that could be used for most of unicellular species, we show that the protein synthesis and cell size are ...
Mohammad Razavi, Seyed Majid Saberi Fathi, Jack Adam Tuszynski   +2 more
doaj   +1 more source