Energy regeneration systems in cell-free protein synthesis in vitro
, 2022 S.67-76Cell-free protein synthesis is a rapidly evolving technology that presents an alternative to conventional recombinant protein production. The technology utilizes cell lysates for ex situ protein synthesis.
Schwedhelm, Ivo +4 more
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Bacterial cell-free expression technology to in vitro systems engineering and optimization
Synthetic and Systems Biotechnology, 2017 Cell-free expression system is a technology for the synthesis of proteins in vitro. The system is a platform for several bioengineering projects, e.g.
Filippo Caschera
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“Cell-Free Synthetic Biology”: Synthetic Biology Meets Cell-Free Protein Synthesis
Methods and Protocols, 2019 Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [...]
Seok Hoon Hong
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Bioengineering, 2023 The XpressCF+® cell-free protein synthesis system is a robust platform for the production of non-natural amino acids containing antibodies, which enable the site-specific conjugation of homogeneous antibody drug conjugates (ADCs) via click chemistry ...
Jeffrey Hanson +15 more
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Protein changes associated with embryonic stem cell differentiation to vascular smooth muscle cells [PDF]
, 2006 PhDEmbryonic stem (ES) cells can differentiate into many different cell lines, including vascular smooth muscle cells (SMCs). The aim of this project is to characterize protein changes during this differentiation process.
Yin, Xiaoke
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Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids. [PDF]
PLoS ONE, 2012 The use of magnetic bead-immobilized DNA as movable template for cell-free protein synthesis has been investigated. Magnetic microbeads containing chemically conjugated plasmids were used to direct cell-free protein synthesis, so that protein generation ...
Ka-Young Lee +3 more
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The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway [PDF]
, 2009 © 2009 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,.
Jacquier, N +10 more
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Versatile cell-free protein synthesis systems based on chinese hamster ovary cells
, 2022 S.289-308We present an alternative production platform for the synthesis of complex proteins. Apart from conventionally applied protein production using engineered mammalian cell lines, this protocol describes the preparation and principle of cell-free ...
Thoring, L., Kubick, S.
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eLifeWhen Escherichia coli ribosomes are assembled in vitro, manipulation of incubation temperature and magnesium ion concentration has been an essential procedure, which is a crucial step for the assembly of active large subunits.
Aya Sato +4 more
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Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery
BioTech, 2021 We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus ...
Marina Snapyan +5 more
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