Improved cell-free RNA and protein synthesis system. [PDF]
PLoS ONE, 2014 Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer new
Jun Li +3 more
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The 89,000-Mr murine cytomegalovirus immediate-early protein stimulates c-fos expression and cellular DNA synthesis [PDF]
, 1988 The immediate-early (IE) genes of murine cytomegalovirus (MCMV) are expressed in the absence of prior viral protein synthesis and regulate the transcription of MCMV early genes. The effect of MCMV IE genes on growth induction was studied.
Philipson, L. +11 more
core +1 more source
Bacterial cell-free expression technology to in vitro systems engineering and optimization
Synthetic and Systems Biotechnology, 2017 Cell-free expression system is a technology for the synthesis of proteins in vitro. The system is a platform for several bioengineering projects, e.g.
Filippo Caschera
doaj +1 more source
Cell-free Synthesis of Pea Seed Proteins [PDF]
Plant Physiology, 1977 Both polysomes and polysomal RNA, isolated from cotyledons of ripening pea (Pisum sativum) seeds and supplemented respectively with wheat germ S-100 and S-30 fractions, were used to program the cell-free synthesis of polypeptides. The relationship of these polypeptide products to seed storage proteins has been investigated. When fractionated on sucrose
T J, Higgins, D, Spencer
openaire +2 more sources
RNA:protein ratio of the unicellular organism as a characteristic of phosphorous and nitrogen stoichiometry and of the cellular requirement of ribosomes for protein synthesis [PDF]
, 2006 Background Mean phosphorous:nitrogen (P:N) ratios and relationships of P:N ratios with the growth rate of organisms indicate a surprising similarity among and within microbial species, plants, and insect herbivores.
Karpinets, Tatiana V. +8 more
core +1 more source
Bioengineering, 2023 The XpressCF+® cell-free protein synthesis system is a robust platform for the production of non-natural amino acids containing antibodies, which enable the site-specific conjugation of homogeneous antibody drug conjugates (ADCs) via click chemistry ...
Jeffrey Hanson +15 more
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Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. [PDF]
, 2014 Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of
Taylor, Peter W +22 more
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Signaling of angiotensin II-induced vascular protein synthesis in conduit and resistance arteries in vivo [PDF]
, 2004 <b>Background</b> From in vitro studies, it has become clear that several signaling cascades are involved in angiotensin II-induced cellular hypertrophy.
Martens Fabrice MAC +23 more
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Translate to divide: сontrol of the cell cycle by protein synthesis [PDF]
, 2015 Protein synthesis underpins much of cell growth and, consequently, cell multiplication. Understanding how proliferating cells commit and progress into the cell cycle requires knowing not only which proteins need to be ...
Aramayo, Rodolfo +3 more
core +1 more source
Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids. [PDF]
PLoS ONE, 2012 The use of magnetic bead-immobilized DNA as movable template for cell-free protein synthesis has been investigated. Magnetic microbeads containing chemically conjugated plasmids were used to direct cell-free protein synthesis, so that protein generation ...
Ka-Young Lee +3 more
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