Results 41 to 50 of about 1,006,135 (293)

Cell-Free Protein Synthesis: Chassis toward the Minimal Cell

Cells, 2019
The quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology.
Ke Yue, Yiyong Zhu, Lei Kai
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Cell‐free protein synthesis [PDF]

The FEBS Journal, 2006
Cell-free protein synthesis using cell extracts from Escherichia coli, wheat germ and rabbit reticulocytes has been used for over 40 years to produce small amounts of radiolabeled proteins for identification of gene products and other applications. In the E.
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Cell-free protein synthesis [PDF]

, 2014
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
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Single-step in vitro reconstitution of the Escherichia coli ribosome mediated by two GTPase factors, EngA and ObgE

eLife
When Escherichia coli ribosomes are assembled in vitro, manipulation of incubation temperature and magnesium ion concentration has been an essential procedure, which is a crucial step for the assembly of active large subunits.
Aya Sato, Weng Yu Lai, Yusuke Sakai, Keiko Masuda, Yoshihiro Shimizu   +4 more
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The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins

Frontiers in Bioengineering and Biotechnology, 2022
The ongoing pandemic caused by the novel coronavirus (SARS-CoV-2) has led to more than 445 million infections and the underlying disease, COVID-19, resulted in more than 6 million deaths worldwide.
Franziska Ramm, Franziska Ramm, Srujan K. Dondapati, Hoai Anh Trinh, Hoai Anh Trinh, Dana Wenzel, Ruben M. Walter, Ruben M. Walter, Anne Zemella, Stefan Kubick, Stefan Kubick, Stefan Kubick   +11 more
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Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics

Synthetic and Systems Biotechnology, 2018
High-sensitivity mass spectrometry approaches using selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) methods are powerful tools for targeted quantitative proteomics-based investigation of dynamics in specific biological systems ...
Ryohei Narumi, Keiko Masuda, Takeshi Tomonaga, Jun Adachi, Hiroki R. Ueda, Yoshihiro Shimizu   +5 more
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Cell-free Synthesis of Adenovirus Coat Proteins

Journal of Biological Chemistry, 1974
Abstract Messenger RNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Four of the polypeptides obtained in this reaction corresponded to the virion coat components hexon, IIIa, penton base, and fiber. The following criteria were
L, Eron, R, Callahan, H, Westphal
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Cell-free synthesis of measles virus proteins [PDF]

Journal of Virology, 1979
Polyadenylated mRNA extracted from cytoplasm of measles virus-infected Vero cells was translated in a cell-free system. Three of the polypeptides obtained corresponded to nucleocapsid protein, phosphoprotein, and membrane protein of measles virions. A fourth polypeptide, present in measles virus-infected cells, could be generated by addition of Vero ...
J, Sprague, L J, Eron, A S, Seifried, J B, Milstien   +3 more
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Rational improvement of cell-free protein synthesis

New Biotechnology, 2011
Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was used.
Anders, Pedersen, Kristofer, Hellberg, Johan, Enberg, B Göran, Karlsson   +3 more
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Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

Synthetic and Systems Biotechnology, 2019
An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor ...
J. Porter Hunt, Emily Long Zhao, Mehran Soltani, Madison Frei, J. Andrew D. Nelson, Bradley C. Bundy   +5 more
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