Results 41 to 50 of about 13,230,245 (270)

Applications of cell free protein synthesis in protein design. [PDF]

Protein Sci
AbstractIn protein design, the ultimate test of success is that the designs function as desired. Here, we discuss the utility of cell free protein synthesis (CFPS) as a rapid, convenient and versatile method to screen for activity. We champion the use of CFPS in screening potential designs.
Thornton EL, Paterson SM, Stam MJ, Wood CW, Laohakunakorn N, Regan L.   +5 more
europepmc   +3 more sources

RNA:protein ratio of the unicellular organism as a characteristic of phosphorous and nitrogen stoichiometry and of the cellular requirement of ribosomes for protein synthesis [PDF]

, 2006
Background Mean phosphorous:nitrogen (P:N) ratios and relationships of P:N ratios with the growth rate of organisms indicate a surprising similarity among and within microbial species, plants, and insect herbivores.
Karpinets, Tatiana V., Greenwood, Duncan J., Greenwood Duncan J, Sams Carl E, Greenwood , Duncan J., Ammons John T, Sams, Carl E., Ammons, John T., Karpinets Tatiana V   +8 more
core   +1 more source

Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics

Synthetic and Systems Biotechnology, 2018
High-sensitivity mass spectrometry approaches using selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) methods are powerful tools for targeted quantitative proteomics-based investigation of dynamics in specific biological systems ...
Ryohei Narumi, Keiko Masuda, Takeshi Tomonaga, Jun Adachi, Hiroki R. Ueda, Yoshihiro Shimizu   +5 more
doaj   +1 more source

The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins

Frontiers in Bioengineering and Biotechnology, 2022
The ongoing pandemic caused by the novel coronavirus (SARS-CoV-2) has led to more than 445 million infections and the underlying disease, COVID-19, resulted in more than 6 million deaths worldwide.
Franziska Ramm, Franziska Ramm, Srujan K. Dondapati, Hoai Anh Trinh, Hoai Anh Trinh, Dana Wenzel, Ruben M. Walter, Ruben M. Walter, Anne Zemella, Stefan Kubick, Stefan Kubick, Stefan Kubick   +11 more
doaj   +1 more source

Cell‐free protein synthesis [PDF]

The FEBS Journal, 2006
Cell-free protein synthesis using cell extracts from Escherichia coli, wheat germ and rabbit reticulocytes has been used for over 40 years to produce small amounts of radiolabeled proteins for identification of gene products and other applications. In the E.
openaire   +2 more sources

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. [PDF]

, 2014
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of
Taylor, Peter W, Paulin, S, Roper, David I, Garcia-Lara, J, Roper, DI, Taylor, PW, Galley, Nicola F., Paulin, Sarah, Rosado, H, Foster, SJ, Taylor, Peter W., Dafforn, TR, Dafforn, Tim, Dafforn, Timothy R, Galley, Nicola F, Jamshad, Mohammed, Rosado, Helena, Foster, Simon J, Garcia-Lara, Jorge, Jamshad, M, Galley, NF, Foster, Simon J., Roper, David I.   +22 more
core   +1 more source

Signaling of angiotensin II-induced vascular protein synthesis in conduit and resistance arteries in vivo [PDF]

, 2004
<b>Background</b> From in vitro studies, it has become clear that several signaling cascades are involved in angiotensin II-induced cellular hypertrophy.
Martens Fabrice MAC, Fabrice MAC Martens, Touyz, R.M., Daigle, Christine, Martens, Fabrice, Touyz Rhian M, Dao Huy, Christine Daigle, Huy Hao Dao, Daphné Girardot, Rhian M Touyz, Moreau Pierre, Girardot, D., Daigle, C., Dao, H., Moreau, P., Girardot, Daphné, Martens, F.M.A.C., Dao, Huy, Daigle Christine, Moreau, Pierre, Girardot Daphné, Pierre Moreau, Touyz, Rhian   +23 more
core   +2 more sources

The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts [PDF]

, 1991
The sites of nascent DNA synthesis were compared with the distribution of the proliferating cell nuclear antigen (PCNA) in S-phase nuclei of human diploid fibroblasts (HDF) by two in vitro techniques.
Kill, IR, Campbell, KHS, Hutchison, CJ, Bridger, JM, Maldonado-Codina, G   +4 more
core   +6 more sources

Modeling Cell-Free Protein Synthesis Systems—Approaches and Applications

Frontiers in Bioengineering and Biotechnology, 2020
In vitro systems are ideal setups to investigate the basic principles of biochemical reactions and subsequently the bricks of life. Cell-free protein synthesis (CFPS) systems mimic the transcription and translation processes of whole cells in a ...
Jan Müller, Martin Siemann-Herzberg, Ralf Takors   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source