Results 61 to 70 of about 13,230,245 (270)

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Cell-free Synthesis of Pea Seed Proteins [PDF]

Plant Physiology, 1977
Both polysomes and polysomal RNA, isolated from cotyledons of ripening pea (Pisum sativum) seeds and supplemented respectively with wheat germ S-100 and S-30 fractions, were used to program the cell-free synthesis of polypeptides. The relationship of these polypeptide products to seed storage proteins has been investigated. When fractionated on sucrose
T J, Higgins, D, Spencer
openaire   +2 more sources

SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging

Nature Communications
Light scattering in the skull limits optical access to the brain. Here we present SeeThrough, a skull-clearing technique that enables simple, high-resolution, and minimally-invasive brain imaging without skull removal.
Xinyi Liu, Motokazu Uchigashima, Ikumi Oomoto, Yoshihito Saito, Hitoshi Uchida, Shinya Oginezawa, Keiko Masuda, Daisuke Satoh, Manabu Abe, Kenji Sakimura, Yoshihiro Shimizu, Masanori Murayama, Kazuki Tainaka, Takayasu Mikuni   +13 more
doaj   +1 more source

Cell-Free Metabolic Engineering: Recent Developments and Future Prospects

Methods and Protocols, 2019
Due to the ongoing crises of fossil fuel depletion, climate change, and environmental pollution, microbial processes are increasingly considered as a potential alternative for cleaner and more efficient production of the diverse chemicals required for ...
Hye Jin Lim, Dong-Myung Kim
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Cell-free Synthesis of Adenovirus Coat Proteins

Journal of Biological Chemistry, 1974
Abstract Messenger RNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Four of the polypeptides obtained in this reaction corresponded to the virion coat components hexon, IIIa, penton base, and fiber. The following criteria were
L, Eron, R, Callahan, H, Westphal
openaire   +2 more sources

Cell-free protein synthesis in hydrogel materials [PDF]

We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the ...
Whitfield CJ, Banks AM, Goodchild SA, Fulton DA, Dura G, Howard TP, Fieldsend JE, Love J   +7 more
core   +4 more sources

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Rational improvement of cell-free protein synthesis

New Biotechnology, 2011
Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was used.
Anders, Pedersen, Kristofer, Hellberg, Johan, Enberg, B Göran, Karlsson   +3 more
openaire   +2 more sources

Cell-free synthesis of measles virus proteins [PDF]

Journal of Virology, 1979
Polyadenylated mRNA extracted from cytoplasm of measles virus-infected Vero cells was translated in a cell-free system. Three of the polypeptides obtained corresponded to nucleocapsid protein, phosphoprotein, and membrane protein of measles virions. A fourth polypeptide, present in measles virus-infected cells, could be generated by addition of Vero ...
J, Sprague, L J, Eron, A S, Seifried, J B, Milstien   +3 more
openaire   +2 more sources