Results 11 to 20 of about 13,230,245 (270)
Cell-free protein synthesis in hydrogel materials [PDF]
Chemical Communications, 2020 Fabrication of macro-scale polysaccharide, proteinaceous, micellular and covalently crosslinked hydrogels for housing cell-free protein synthesis reactions.
Colette J. Whitfield +7 more
openaire +6 more sources
, 2020 Proteins are the main source of drug targets and some of them possess therapeutic potential themselves. Among them, membrane proteins constitute approximately 50% of the major drug targets. In the drug discovery pipeline, rapid methods for producing different classes of proteins in a simple manner with high quality are important for structural and ...
Dondapati, Srujan Kumar +3 more
openaire +3 more sources
Silk fibroin as an additive for cell-free protein synthesis
Synthetic and Systems Biotechnology, 2020 Cell-free systems contain many proteins and metabolites required for complex functions such as transcription and translation or multi-step metabolic conversions.
Marilyn S. Lee +5 more
doaj +3 more sources
Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins
Methods and Protocols, 2019 Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the
Xing Jin +2 more
doaj +3 more sources
Life, 2018 Membrane proteins are important drug targets which play a pivotal role in various cellular activities. However, unlike cytosolic proteins, most of them are difficult-to-express proteins.
Belay Gessesse +4 more
doaj +3 more sources
Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs [PDF]
, 1989 The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy.
Kill, IR, Hutchison, C
core +6 more sources
Light-controlled cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides [PDF]
, 2022 Cell-free expression of a gene to protein has become a vital tool applied in DNA nanodevices and synthetic cells. Antisense oligonucleotides are often used to induce gene knockdown; however, they have been underexplored for cell-free applications ...
Denis, Hartmann, Michael, Booth
core +2 more sources
Cancer Medicine, 2023 Purpose The pathogenesis of cancers depends on the molecular background of each individual patient. Therefore, verifying as many biomarkers as possible and clarifying their relationships with each disease status would be very valuable.
Keiko Kasahara +9 more
doaj +1 more source
Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems. [PDF]
PLoS ONE, 2016 Cell-free protein synthesis, which mimics the biological protein production system, allows rapid expression of proteins without the need to maintain a viable cell.
Jurek Failmezger +4 more
doaj +1 more source
Optimising protein synthesis in cell‐free systems, a review
Engineering Biology, 2021 Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology.
Angelo C. Batista +3 more
doaj +1 more source