Results 11 to 20 of about 1,006,135 (293)
Cell-free protein synthesis in hydrogel materials [PDF]
Chemical Communications, 2020 Fabrication of macro-scale polysaccharide, proteinaceous, micellular and covalently crosslinked hydrogels for housing cell-free protein synthesis reactions.
Colette J. Whitfield +7 more
openaire +7 more sources
Physical stimuli-responsive cell-free protein synthesis [PDF]
Synthetic and Systems Biotechnology, 2020 Cell-free protein synthesis has been developed as a critical platform in synthetic biology. Unlike the cell-based synthesis system, cell-free system activates transcriptional and translational mechanisms in vitro, and can control protein synthesis by ...
Junzhu Yang, Yuan Lu
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Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 One of the main advantages of a cell-free synthesis system is that the synthetic machinery of cells can be modularized and re-assembled for desired purposes.
Yu-Jin Park, Dong-Myung Kim
doaj +2 more sources
Silk fibroin as an additive for cell-free protein synthesis
Synthetic and Systems Biotechnology, 2020 Cell-free systems contain many proteins and metabolites required for complex functions such as transcription and translation or multi-step metabolic conversions.
Marilyn S. Lee +5 more
doaj +3 more sources
Cell-Free Protein Synthesis [PDF]
, 2012 The Nobel Prize in Medicine 1968 for interpretation of the genetic code and its function in protein synthesis and in Chemistry 2009 for studies of the structure and function of the ribosome highlighted the ground-breaking experiment performed on May 15 ...
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Light-controlled cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides [PDF]
, 2022 Cell-free expression of a gene to protein has become a vital tool applied in DNA nanodevices and synthetic cells. Antisense oligonucleotides are often used to induce gene knockdown; however, they have been underexplored for cell-free applications ...
Denis, Hartmann, Michael, Booth
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Cancer Medicine, 2023 Purpose The pathogenesis of cancers depends on the molecular background of each individual patient. Therefore, verifying as many biomarkers as possible and clarifying their relationships with each disease status would be very valuable.
Keiko Kasahara +9 more
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Versatile cell-free protein synthesis systems based on chinese hamster ovary cells [PDF]
, 2022 S.289-308We present an alternative production platform for the synthesis of complex proteins. Apart from conventionally applied protein production using engineered mammalian cell lines, this protocol describes the preparation and principle of cell-free ...
Thoring, L., Kubick, S.
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Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems. [PDF]
PLoS ONE, 2016 Cell-free protein synthesis, which mimics the biological protein production system, allows rapid expression of proteins without the need to maintain a viable cell.
Jurek Failmezger +4 more
doaj +1 more source
Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction. [PDF]
, 2023 CD1E, one of the most important glycolipid antigens on T cell membranes, is required for glycolipid antigen presentation on the cell surface. Cell-based recombinant expression systems have many limitations for synthesizing transmembrane proteins such as ...
Tang, Y +9 more
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