Results 41 to 50 of about 6,347,699 (399)

Aptamer-based cell-free detection system to detect target protein

Synthetic and Systems Biotechnology, 2021
Biomarkers of disease, especially protein, show great potential for diagnosis and prognosis. For detecting a certain protein, a binding assay implementing antibodies is commonly performed.
Junhong Chen, Xiaoyan Zhuang, Jiyang Zheng, Ruofan Yang, Fei Wu, Aihui Zhang, Baishan Fang   +6 more
doaj   +1 more source

Breast Milk from Tanzanian Women has Divergent Effects on Cell-Free and Cell-Associated HIV-1 Infection in Vitro. [PDF]

, 2012
Transmission of HIV-1 during breastfeeding is a significant source of new pediatric infections in sub-Saharan Africa. Breast milk from HIV-positive mothers contains both cell-free and cell-associated virus; however, the impact of breast milk on HIV-1 ...
Ruth I Connor, Alexandra L Howell, Molly L Housman, Lee, F.V, Housman, M.L., Zain-Ul-Abideen Muhammad, Frederick V Lee, Zain-Ul-Abideen, M., Connor, R.I, Connor Ruth I., Zain-Ul-Abideen, Muhammad, Lyimo, M.A., Howell, Alexandra L, Lyimo, Magdalena A, Magdalena A Lyimo, Howell Alexandra L., Housman, Molly L, Mosi, M.N, Muhammad Zain-Ul-Abideen, Howell, A.L, Lee, Frederick V, Mosi, Matilda Ngarina, Matilda Ngarina Mosi, Connor, Ruth I, Lyimo Magdalena A., Housman Molly L., Mosi Matilda Ngarina, Lee Frederick V.   +27 more
core   +1 more source

Auxin Action in a Cell-Free System [PDF]

Current Biology, 2003
The plant hormone auxin regulates diverse aspects of plant growth and development [1]. Despite its importance, the mechanisms of auxin action remain poorly understood. In particular, the identities of the auxin receptor and other signaling proteins are unknown.
Dharmasiri, Nihal, Dharmasiri, Sunethra, Jones, Alan M., Estelle, Mark   +3 more
openaire   +2 more sources

A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]

, 2011
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
M. O. Riehle, J.R. Higginson (7130108), Higginson, J.R., Donoghue, P.S., T. Sun, Nikolaj Gadegaard (1292763), J. R. Higginson, N. Gadegaard, Riehle, M.O., Mathis O. Riehle (1292769), Barnett, S.C., Sun, T., Tao Sun (1255119), Peter S. Donoghue (7128581), S. C. Barnett, Gadegaard, N., P. S. Donoghue, Susan C. Barnett (7128590)   +17 more
core   +1 more source

Exploiting cell‐free systems: Implementation and debugging of a system of biotransformations [PDF]

Biotechnology and Bioengineering, 2010
AbstractThe orchestration of a multitude of enzyme catalysts allows cells to carry out complex and thermodynamically unfavorable chemical conversions. In an effort to recruit these advantages for in vitro biotransformations, we have assembled a 10‐step catalytic system—a system of biotransformations (SBT)—for the synthesis of unnatural monosaccharides ...
Bujara M, Schümperli M, Billerbeck S, Heinemann M, Panke S   +4 more
openaire   +4 more sources

Physical stimuli-responsive cell-free protein synthesis

Synthetic and Systems Biotechnology, 2020
Cell-free protein synthesis has been developed as a critical platform in synthetic biology. Unlike the cell-based synthesis system, cell-free system activates transcriptional and translational mechanisms in vitro, and can control protein synthesis by ...
Junzhu Yang, Yuan Lu
doaj   +1 more source

Visual histological grading system for the evaluation of in vitro-generated neocartilage [PDF]

, 2006
Here we present the development of a visual evaluation system for routine assessment of in vitro-engineered cartilaginous tissue. Neocartilage was produced by culturing human articular chondrocytes in pellet culture systems or in a scaffold-free ...
Fitzsimmons, James S., O'Driscoll, Shawn, O`Driscoll, Shawn, Grogan, Shawn Patrick, Winkelmann, Verena, Martin, Ivan, Rieser, Franz, Barbero, Andrea, Fitzsimmons, James S, Mainil-Varlet, Pierre   +9 more
core   +1 more source

Spectral Efficiency of Network-Assisted Full-Duplex for Cell-Free Massive MIMO System Under Pilot Contamination

IEEE Access, 2021
A cell-free massive multiple-input multiple-output (MIMO) network-assisted full-duplex (NAFD) system has been proposed to satisfy the exploding demand of higher data transmission speed and more efficient communication.
Yanfeng Hu, Hongwu Ge, Haidong Wang, Dongming Wang   +3 more
doaj   +1 more source

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors [PDF]

, 2011
Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs ...
Braun, Dieter, Shuguang Zhang, Baaske Philipp, Moran Jerabek-Willemsen, Geissler, Sandra, Duhr, Stefan, Duhr Stefan, Corin, Karolina A., Sandra Geissler, Wienken Christoph J., Christoph J. Wienken, Geissler Sandra, Jerabek-Willemsen, Moran, Christoph J Wienken, Zhang Shuguang, Stefan Duhr, Emily Brown, Wang Xiaoqiang, Philipp Baaske, Deepali B. Ravel, Brown, Emily, Song Junyao, Xiaoqiang Wang, Braun Dieter, Karolina Corin, Junyao Song, Wang, Xiaoqiang, Dieter Braun, Baaske, Philipp, Wienken, Christoph J., Ravel Deepali B., Corin, Karolina, Ravel, Deepali B., Brown Emily, Song, Junyao, Jerabek-Willemsen Moran, Corin Karolina, Zhang, Shuguang, Deepali B Ravel, Brown, Emily E., Ra Geissler   +40 more
core   +1 more source

Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways

Microorganisms, 2022
As a fast and reliable technology with applications in diverse biological studies, cell-free protein synthesis has become popular in recent decades. The cell-free protein synthesis system can be considered a complex chemical reaction system that is also ...
Anwesha Purkayastha, Kathirvel Iyappan, Taek Jin Kang   +2 more
doaj   +1 more source