Results 11 to 20 of about 2,292,973 (264)
OnePot PURE Cell-Free System [PDF]
Journal of Visualized Experiments, 2021 The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In comparison, a home-made approach can be customized based on user needs.
Grasemann, Laura +3 more
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Improved cell-free RNA and protein synthesis system. [PDF]
PLoS ONE, 2014 Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer new
Jun Li +3 more
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Auxin Action in a Cell-Free System [PDF]
Current Biology, 2003 The plant hormone auxin regulates diverse aspects of plant growth and development [1]. Despite its importance, the mechanisms of auxin action remain poorly understood. In particular, the identities of the auxin receptor and other signaling proteins are unknown.
Dharmasiri, Nihal +3 more
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Supramolecular protein assembly in cell-free protein synthesis system
Bioresources and Bioprocessing, 2022 Protein-based biomaterials have the characteristics of stability and biocompatibility. Based on these advantages, various bionic materials have been manufactured and used in different fields.
Zhixia Li +6 more
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Akaby-Cell-free protein expression system for linear templates.
PLoS ONE, 2022 Cell-free protein expression is increasingly becoming popular for biotechnology, biomedical and research applications. Among cell-free systems, the most popular one is based on Escherichia coli (E. coli). Endogenous nucleases in E.
Wakana Sato +6 more
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Zaporožskij Medicinskij Žurnal, 2019 The paper presents the results of the study on proliferation and biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis under the influence of cell-free extracts obtained by the author’s method and containing derivatives of probiotic ...
O. V. Knysh +3 more
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Aptamer-based cell-free detection system to detect target protein
Synthetic and Systems Biotechnology, 2021 Biomarkers of disease, especially protein, show great potential for diagnosis and prognosis. For detecting a certain protein, a binding assay implementing antibodies is commonly performed.
Junhong Chen +6 more
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Geometric Effect for Biological Reactors and Biological Fluids
Bioengineering, 2018 As expressed “God made the bulk; the surface was invented by the devil„ by W. Pauli, the surface has remarkable properties because broken symmetry in surface alters the material properties.
Kazusa Beppu +3 more
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Physical stimuli-responsive cell-free protein synthesis
Synthetic and Systems Biotechnology, 2020 Cell-free protein synthesis has been developed as a critical platform in synthetic biology. Unlike the cell-based synthesis system, cell-free system activates transcriptional and translational mechanisms in vitro, and can control protein synthesis by ...
Junzhu Yang, Yuan Lu
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THE SYNTHESIS OF HEMOGLOBIN IN A CELL-FREE SYSTEM [PDF]
Proceedings of the National Academy of Sciences, 1958 The microsomal particles (1) have been implicated as the major sites of protein synthesis within the cells of a variety of tissues. The studies of Borsook, Zamecnik, Hultin, and others, with intact animals and various types of whole cell systems (see review by Askonas et al.
R, Schweet, H, Lamfrom, E, Allen
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