PLoS ONE, 2011 Bacteria, fungi, protozoa, chromista and plants all harbor homologues of Hsp104, a AAA+ ATPase that collaborates with Hsp70 and Hsp40 to promote protein disaggregation and reactivation. Curiously, however, metazoa do not possess an Hsp104 homologue. Thus,
James Shorter
openalex +3 more sources
A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins [PDF]
BioengineeringCell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation ...
Jeffrey L. Schloßhauer +3 more
doaj +2 more sources
The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare
Biochemical Engineering Journal, 2021 Caroline E. Copeland +3 more
semanticscholar +3 more sources
Cell lysates and egg white create homeostatic microenvironment for gene expression in cell-free system [PDF]
Synthetic and Systems Biotechnology, 2018 Homeostasis widely exists in living systems, and plays essential roles for maintaining normal physiological activities, enabling to preserve their functionalities against variations.
Yang Liu, Dayong Yang
doaj +2 more sources
Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses [PDF]
Scientific Reports, 2017 Membrane proteins are key elements in cell-mediated processes. In particular, G protein-coupled receptors (GPCRs) have attracted increasing interest since they affect cellular signaling.
Anne Zemella +5 more
doaj +2 more sources
SMIM1, carrier of the Vel blood group, is a tail-anchored transmembrane protein and readily forms homodimers in a cell-free system. [PDF]
Biosci Rep, 2020 Nylander A +4 more
europepmc +3 more sources
RIS-Aided Cell-Free Massive MIMO Systems for 6G: Fundamentals, System Design, and Applications [PDF]
Proceedings of the IEEE, 2023 An introduction of intelligent interconnectivity for people and things has posed higher demands and more challenges for sixth-generation (6G) networks, such as high spectral efficiency and energy efficiency (EE), ultralow latency, and ultrahigh ...
Enyu Shi +7 more
semanticscholar +1 more source
Translation initiation consistency between in vivo and in vitro bacterial protein expression systems
Frontiers in Bioengineering and Biotechnology, 2023 Strict on-demand control of protein synthesis is a crucial aspect of synthetic biology. The 5′-terminal untranslated region (5′-UTR) is an essential bacterial genetic element that can be designed for the regulation of translation initiation.
Jiaojiao Li +10 more
doaj +1 more source
Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals [PDF]
The Ukrainian Biochemical Journal, 2020 The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was ...
A. K. Gulevskyy +2 more
doaj +1 more source
An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation
eLife, 2022 The ring-like ATPase complexes in the AAA+ family perform diverse cellular functions that require coordination between the conformational transitions of their individual ATPase subunits (Erzberger and Berger, 2006; Puchades et al., 2020).
Rui Fang +3 more
doaj +1 more source