Results 201 to 210 of about 1,006,135 (293)

Rapid screening and scaled manufacture of immunogenic virus-like particles in a tobacco BY-2 cell-free protein synthesis system. [PDF]

Front Immunol, 2023
Armero-Gimenez J, Wilbers R, Schots A, Williams C, Finnern R.   +4 more
europepmc   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants. [PDF]

ACS Synth Biol
Thornton EL, Boyle JT, Laohakunakorn N, Regan L.   +3 more
europepmc   +1 more source

Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases. [PDF]

Antioxidants (Basel), 2022
Schramm M, Friedrich S, Schmidtke KU, Kiebist J, Panzer P, Kellner H, Ullrich R, Hofrichter M, Scheibner K.   +8 more
europepmc   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Engineering At-Home Dilution and Filtration Methods to Enable Paper-Based Colorimetric Biosensing in Human Blood with Cell-Free Protein Synthesis. [PDF]

Biosensors (Basel), 2023
Free TJ, Tucker RW, Simonson KM, Smith SA, Lindgren CM, Pitt WG, Bundy BC.   +6 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Developing an <i>E. coli</i>-Based Cell-Free Protein Synthesis System for Artificial Spidroin Production and Characterization. [PDF]

ACS Synth Biol
Huang CY, Wang RC, Hsu TS, Hung TN, Shen MY, Chang CH, Wu HC.   +6 more
europepmc   +1 more source

An efficient cell-free protein synthesis platform for producing proteins with pyrrolysine-based noncanonical amino acids. [PDF]

Biotechnol J, 2022
Ranji Charna A, Des Soye BJ, Ntai I, Kelleher NL, Jewett MC.   +4 more
europepmc   +1 more source