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Advanced Functional Materials, EarlyView.The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett +19 more
wiley +1 more source
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Cell-free protein synthesis systems
Biotechnology Advances, 1998Cell-free protein synthesis systems enable the direct in vitro expression of proteins from template DNA or RNA. Use of biochemical and bioengineering techniques has greatly improved the yields and productivities of cell-free systems. In some cases, the yields approach the in vivo levels.
Hideo Nakano
exaly +3 more sources
Methods for energizing cell-free protein synthesis
Journal of Bioscience and Bioengineering, 2009The productivity of cell-free protein synthesis depends critically upon the efficiency of ATP regeneration. In addition, the expensive energy sources used in the conventional cell-free protein synthesis systems account for the major part of the overall cost for the expression of proteins.
Dong-Myung Kim, Ho-Cheol Kim
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Cell-free protein synthesis [PDF]
, 2020 Proteins are the main source of drug targets and some of them possess therapeutic potential themselves. Among them, membrane proteins constitute approximately 50% of the major drug targets. In the drug discovery pipeline, rapid methods for producing different classes of proteins in a simple manner with high quality are important for structural and ...
Dondapati, Srujan Kumar +3 more
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Cell-free protein synthesis on a microchip
PROTEOMICS, 2002We evaluated the expression of various proteins by using a cell-free protein synthesis system with a rapid translation system, a microtube, and a microchip technique. Protein expression was successfully achieved with a microfabricated reaction chamber on a plastic chip.
Marl, Tabuchi +3 more
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Arraying proteins by cell-free synthesis
Biomolecular Engineering, 2007Recent advances in life science have led to great motivation for the development of protein arrays to study functions of genome-encoded proteins. While traditional cell-based methods have been commonly used for generating protein arrays, they are usually a time-consuming process with a number of technical challenges.
Mingyue, He, Ming-Wei, Wang
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Cell-Free Protein Synthesis of Metalloproteins
2023Metalloproteins, proteins containing metal atoms or clusters within their structures, are critical for various biological functions across all domains of life. More than hundreds of different types have been discovered, which conduct various roles such as transportation of O2, catalyzing chemical reactions, sensing environmental changes, and relaying ...
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Systems for the Cell-Free Synthesis of Proteins
2011We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given ...
Lei, Kai +8 more
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Cell-free synthesis of zinc-binding proteins
Journal of Structural and Functional Genomics, 2006Cell-free protein synthesis has become one of the standard methods for protein expression. The cell-free method is suitable for the synthesis of a protein that requires a ligand for its enzymatic activity and/or structure formation and stabilization, since it is an open system, which allows us to add the proper ligand to the reaction mixture.
Takayoshi, Matsuda +8 more
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