Results 261 to 270 of about 1,006,135 (293)

SYNAPTOSOMAL PROTEIN SYNTHESIS IN A CELL‐FREE SYSTEM*

Journal of Neurochemistry, 1968
Abstract—Synaptosomes were isolated from cerebral cortex of young rats and incubated with14C‐labelledl‐leucinein vitro. Amino acid incorporation into proteins of the synaptosomal cytoplasm, mitochondria and membrane components was observed. There was no incorporation into proteins of the vesicles.
I G, Morgan, L, Austin
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Selenomethionine incorporation into a protein by cell-free synthesis

Journal of Structural and Functional Genomics, 2002
Multi-wavelength anomalous diffraction phasing is especially useful for high-throughput structure determinations. Selenomethionine substituted proteins are commonly used for this purpose. However, the cytotoxicity of selenomethionine drastically reduces the efficiency of its incorporation in in vivo expression systems. In the present study, an improved
T. Kigawa, E. Yamaguchi-Nunokawa, K. Kodama, T. Matsuda, T. Yabuki, Natsuko Matsuda, R. Ishitani, O. Nureki, S. Yokoyama   +8 more
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Cell-free protein synthesis for biocatalysis

Cell-free protein synthesis (CFPS) serves as an innovation booster in the field of biocatalysis. By integrating CFPS into the design and development of biocatalysts, the discovery, synthesis, and screening of previously untapped enzymes and their engineered variants can be improved.
Siranjeevi, Balakrishnan, Katrin, Rosenthal   +1 more
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Cell-free protein synthesis and assembly on a biochip

Nature Nanotechnology, 2012
Biologically active complexes such as ribosomes and bacteriophages are formed through the self-assembly of proteins and nucleic acids. Recapitulating these biological self-assembly processes in a cell-free environment offers a way to develop synthetic biodevices.
Yael, Heyman, Amnon, Buxboim, Sharon G, Wolf, Shirley S, Daube, Roy H, Bar-Ziv   +4 more
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A novel cell-free protein synthesis system

Journal of Biotechnology, 2004
An efficient cell-free protein synthesis system has been developed using a novel energy-regenerating source. Using the new energy source, 3-phosphoglycerate (3-PGA), protein synthesis continues beyond 2 h. In contrast, the reaction rate slowed down considerably within 30-45 min using a conventional energy source, phosphoenol pyruvate (PEP) under ...
Kalavathy, Sitaraman, Dominic, Esposito, George, Klarmann, Stuart F, Le Grice, James L, Hartley, Deb K, Chatterjee   +5 more
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Cell‐Free Protein Synthesis by Rumen Protozoa

The Journal of Protozoology, 1968
SYNOPSIS. The characteristics of protein synthesis by cell‐free extracts of mixed rumen protozoa have been investigated. ATP,1 GTP, and an energy supply system were necessary for amino acid incorporation which was partially inhibited by cycloheximide but not by chloramphenicol (100 μg/ml).
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Cell-Free Synthesis of Factor VIII Related Protein

Thrombosis and Haemostasis, 1983
SummaryPolyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunopredpitation, the translation product was found to contain 1.58% VIH related protein (VIIIR), which has a molecular weight of 0.26 × 106 ...
Chan, V, Chan, TK
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Cell-free synthesis of ribosomal proteins on polyribosomes

Journal of Molecular Biology, 1971
Abstract Polyribosomes isolated from normally growing Escherichia coli were used to direct the cell-free incorporation of radioactive amino acids into proteins. The proteins synthesized in vitro were characterized by carboxymethyl cellulose column chromatography followed by acrylamide gel electrophoresis. At least 18 ribosomal proteins appear to have
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Protein Synthesis in Cell-Free Systems

1998
Cell-free extracts are widely used to identify and study the proteins encoded by cloned cDNAs, viral genomes or other exogenous mRNA, etc., as well as to investigate transcriptional and translational control. In vitro translation is in many instances the method of choice for the rapid analysis of uncharacterized proteins, provided that the cDNA ...
Tiina Tamm, Erkki Truve
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Universal cell-free protein synthesis

Nature Biotechnology, 2009
Unstructured translation-initiation sequences enable protein synthesis in cell extracts from multiple organisms.
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