Results 181 to 190 of about 3,447,785 (360)
Summary: Human primary cardiomyocytes are a valuable in vitro model for studying human heart physiology and disease. Here, we present a protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion ...
Xun Shi, Yafei Huang, Bingying Zhou
doaj
In vitro culture of human thyroid cells: Methods and application to radiation biology. [PDF]
Richard C. Miller+6 more
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We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring+6 more
wiley +1 more source
Biology of Normal Proliferating Cells In Vitro: Relevance for In Vivo Aging [PDF]
A.H. Bittles
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Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke+2 more
wiley +1 more source
Protein engineering Tutorials in molecular and cell biology [PDF]
M. James C. Crabbe
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Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek+15 more
wiley +1 more source