Results 211 to 220 of about 2,061,986 (268)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Establishment of a Novel Caco-2-Based Cell Culture System for Human Sapovirus Propagation. [PDF]

Genes Cells
Fukuda Y, Ishikawa A, Ishiyama R, Takai-Todaka R, Haga K, Someya Y, Kimura-Someya T, Katayama K.   +7 more
europepmc   +1 more source

Genetic diversity and population structure of the Taigan dog breed

FEBS Open Bio, EarlyView.
The Taigan is a rare sighthound from the mountains of Kyrgyzstan. We used DNA markers to study its genetic diversity and compare it with other sighthound breeds. Our findings show that the Taigan shares close genetic ties with the Kazakh Tazy, suggesting a common ancestry shaped by nomadic traditions and regional adaptation.
Kira Bespalova, Anastassiya Perfilyeva, Мamura Begmanova, Assel Zhaxylykova, Arailym Yerzhan, Kanagat Yergali, Almazbek Akunov, Marat Munarbek uulu, Anna Khamchukova, Almira Amirgaliyeva, Yelena Kuzovleva   +10 more
wiley   +1 more source

Fabrication of a novel 3D-printed perfusion bioreactor for complex cell culture models. [PDF]

Sci Rep
Jun BH, Torrez JE, Ross DJ, Patterson BM, Ishak MO, Rodriguez AM, Harris JF, Davis-Anderson KL.   +7 more
europepmc   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Injectable, Manganese-Labeled Alginate Hydrogels as a Matrix for Longitudinal and Rapidly Retrievable 3D Cell Culture. [PDF]

Int J Mol Sci
Malysz-Cymborska I, Golubczyk D, Walczak P, Stanaszek L, Janowski M.   +4 more
europepmc   +1 more source

A Neutralization Test for Trachoma and Inclusion Blennorrhoea Viruses Grown in HeLa Cell Cultures

, 1962
P. Reeve, Doris M. Graham
openalex   +1 more source

Functional parameters of spermatozoa obtained by a new selection device

FEBS Open Bio, EarlyView.
This study compares density gradient centrifugation and the LensHooke CA0™ device for sperm separation. DGC requires multiple steps, specialized equipment, and technical expertise. In contrast, the CA0 device offers key practical and methodological advantages, resulting in a faster, simpler, and more affordable sperm selection method.
Julio C. Chávez, Paulina Torres, Gabriela Carrasquel‐Martínez, María G. Figueroa‐Méndez, Diana Flores, Lina Villar, Israel Maldonado, Marcela B. Trevino, Claudia Treviño   +8 more
wiley   +1 more source

Bacteriorhodopsin-Based pH Sensor for Cell Culture Condition Regulation. [PDF]

Materials (Basel)
Huang J, Xie S, Fan H, Song C, Zheng Q, Luo D, Zeng Z, Li Z, Lv Y.   +8 more
europepmc   +1 more source

AN OUTBREAK OF EQUINE VIRUS ABOR TION IN TURKEY I. ISOLATION AND IDENTIFICATION OF RHINOPNEUMONlTIS VIRUS IN CELL CULTURES

, 1968
Başkaya Hasan Doğuer
openalex   +2 more sources