Results 221 to 230 of about 3,334,633 (288)

Analysis of Polymer/siRNA Nanoparticle Efficacy and Biocompatibility in 3D Air-Liquid Interface Culture Compared to 2D Cell Culture. [PDF]

Pharmaceutics
Noske S, Krueger M, Ewe A, Aigner A.
europepmc   +1 more source

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

FEBS Open Bio, EarlyView.
We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei, Amir Barzegar Behrooz, Marco Cordani, Mahmoud Aghaei, Negar Azarpira, Daniel J. Klionsky, Saeid Ghavami   +6 more
wiley   +1 more source

In vitro enzymatic and cell culture assays for SARS-CoV-2 main protease interaction with ambenonium. [PDF]

Sci Rep
de Souza JÂ, de Souza Gomes I, de Souza Fernandes L, Andrade LAF, de Souza LÂ, de Almeida Paiva V, Araujo SC, de Lima LHF, Dias RS, de Melo-Minardi RC, da Fonseca FG, de Paula SO, de Azevedo Silveira S.   +12 more
europepmc   +1 more source

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500‐times more potent and odorless alternative to butyrate

FEBS Open Bio, EarlyView.
Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann   +4 more
wiley   +1 more source

In vitro cellular interaction of drug-loaded liposomes with 2D and 3D cell culture of U87-MG cell line. [PDF]

PLoS One
Alsheleh T, Zraikat M, Daoud F, Alqudah DA, Abdelghany S, Abu Siniyeh A, Alshaer W.   +6 more
europepmc   +1 more source

Establishment and biological characterization of radioresistant colorectal cancer cell lines

FEBS Open Bio, EarlyView.
Under ionizing radiation exposure, radiation‐sensitive cancer cells exhibit oxidative stress and DNA damage, while radiation‐resistant cancer cells exhibit strong antioxidant properties and DNA damage repair. Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC).
Tian‐Yin Qu, Qing Dai, Jing Leng, Lin Fang, Jing‐Jing Ma, Rui Chen, Che Chen, Peng‐Fei Ran, Wen‐Wen Zhou, Chang Liu, Huang‐Fei Yu   +10 more
wiley   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Triplicate Dynamic Cell Culture Platform for Enhanced Reproducibility in Anti-Cancer Drug Testing. [PDF]

ACS Biomater Sci Eng
Lu YL, Lin CM, Huang JH.
europepmc   +1 more source

Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, EarlyView.
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik, Deniz Bakkalci, Alexander J. MacRobert, Umber Cheema   +3 more
wiley   +1 more source

Ligand-Independent Spontaneous Activation of Purinergic P2Y₆ Receptor Under Cell Culture Soft Substrate. [PDF]

Cells
Nishimura A, Nishiyama K, Ito T, Mi X, Kato Y, Inoue A, Aoki J, Nishida M.   +7 more
europepmc   +1 more source