Modifications at the C-Terminus To Improve Pyrrole−Imidazole Polyamide Activity in Cell Culture [PDF]
, 2009 Pyrrole−imidazole (Py-Im) hairpin polyamides are a class of small molecule DNA minor groove binding compounds that have been shown to modulate endogenous gene expression in cell culture.
Dervan, Peter B., Jacobs, Claire S.
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Frontiers in Molecular Biosciences, 2022 Type 2 diabetes mellitus (T2DM) is a growing global public health issue, and dipeptidyl peptidase-4 (DPP-4) is a potential therapeutic target in T2DM. Several synthetic anti-DPP-4 medications can be used to treat T2DM. However, because of adverse effects,
Sibhghatulla Shaikh +17 more
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Scaffold-based 3D cell culture models in cancer research
Journal of Biomedical SciencesThree-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems.
Waad H. Abuwatfa, W. Pitt, G. Husseini
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Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion. [PDF]
, 1975 Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of
Armstrong, Peter, Lackie, J
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Transcriptional landscape of epithelial and immune cell populations revealed through FACS-seq of healthy human skin. [PDF]
, 2017 Human skin consists of multiple cell types, including epithelial, immune, and stromal cells. Transcriptomic analyses have previously been performed from bulk skin samples or from epithelial and immune cells expanded in cell culture.
Ahn, Richard S +9 more
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Improving the metabolic fidelity of cancer models with a physiological cell culture medium
Science Advances, 2019 Plasmax, a physiological cell culture medium, prevents metabolic artifacts imposed on cancer cells by commonly used media. Currently available cell culture media may not reproduce the in vivo metabolic environment of tumors.
J. Vande Voorde +9 more
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In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines
Czech Journal of Food Sciences, 2019 In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity.
Cetin Akca +5 more
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Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling
World Journal of Stem Cells, 2019 Three-dimensional (3D) culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.
Nipha Chaicharoenaudomrung +2 more
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Characterizing steady states of genome-scale metabolic networks in continuous cell cultures
, 2017 We present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation.
Fernandez-de-Cossio-Diaz, Jorge +2 more
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Evidence-based guidelines for controlling pH in mammalian live-cell culture systems
Communications Biology, 2019 A fundamental variable in culture medium is its pH, which must be controlled by an appropriately formulated buffering regime, since biological processes are exquisitely sensitive to acid–base chemistry.
J. Michl, K. Park, P. Swietach
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