Results 211 to 220 of about 579,141 (263)

Aseptic Technique for Cell Culture

Current Protocols in Cell Biology, 1998
AbstractThis unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch‐all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality,
openaire   +2 more sources

Basic Techniques in Mammalian Cell Tissue Culture

Current Protocols in Toxicology, 2015
AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy, Phelan, Kristin M, May
openaire   +3 more sources

Essential Techniques of Cancer Cell Culture

2003
A primary cell culture is the initial culture set up directly from a body tissue. Primary cancer cultures can be initiated and derived from a variety of tissue types such as solid tumor fragments (primary or metastatic) or cell suspensions, for example, aspirates, including peritoneal ascites or pleural effusions.
Kenneth G, MacLeod, Simon P, Langdon
openaire   +2 more sources

A simple marker technique for cells in culture

Experimental Cell Research, 1964
To study the interaction of two morphologically similar populations of living cells, it is necessary to distinguish them with some suitable marker. A characteristic karyotype may be used for this purpose and has the advantage that it persists during cell multiplication, but it is not possible to make direct differential counts on the living cells.
openaire   +2 more sources

Overview of Cell and Tissue Culture Techniques

Current Protocols in Pharmacology, 1999
AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
openaire   +3 more sources

Techniques for Mammalian Cell Tissue Culture

Current Protocols in Human Genetics, 1994
AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
openaire   +7 more sources

Cell Culture Techniques

2011
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
openaire   +1 more source

General Techniques of Cell Culture

1997
Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a
Maureen A. Harrison, Ian F. Rae
openaire   +1 more source

Techniques for Culturing Arterial Endothelial Cells

1977
Lazzarini (3) was one of the first to make use of the methods of tissue culture in the study of the nature of cells of blood vessels. His pioneer work was continued and extended by Pollack and his group (5). Like any other new approach this also had been vigorously discussed until recently.
openaire   +2 more sources

A simplified technique for the culture of amniotic fluid cells

The Journal of Pediatrics, 1971
Summary Based on studies of 40 consecutive amnioticfluid specimens, it was concluded that the most reliable and efficient technique for the culture of amniotic fluid cells is to utilize the amniotic fluid itself as the culture medium. Neither additives nor manipulation of the fluid are necessary; even gentle centrifugation and resuspension may ...
C, Gray, R G, Davidson, M M, Cohen
openaire   +2 more sources