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Studies of renal cell function using cell culture techniques
American Journal of Physiology-Renal Physiology, 1980Cell culture, a powerful tool for the study of cell biology, offers advantages for the study of renal cell function. Epithelial cells derived from a variety of organs, including the kidney, form oriented epithelial sheets in culture that have many structural characteristics (microvilli, tight junctions) of epithelia in situ.
J S, Handler, F M, Perkins, J P, Johnson
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Practical Cell Culture Techniques
1992Harold/Walz: Basic Techniques for Cell Culturing. Barnstable: Identification of Cell Types in Neural Cultures. Bottenstein: Environmental Influences on Cells in Culture. Manthorpe et al.: Detection and Analysis of Growth Factors Affecting Neural Cells. Ure/Acheson: The Role of Cell Adhesion Molecules in Neurite Growth.
Alan A. Boulton +2 more
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Fabrication techniques of biomimetic scaffolds in three‐dimensional cell culture: A review
Journal of Cellular Physiology, 2020In the last four decades, several researchers worldwide have routinely and meticulously exercised cell culture experiments in two‐dimensional (2D) platforms.
A. K. Badekila, S. Kini, Amit K Jaiswal
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Germ Cell Tumor Cell Culture Techniques
2020Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture.
John T, Lafin +2 more
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Aseptic Technique for Cell Culture
Current Protocols in Cell Biology, 1998AbstractThis unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch‐all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality,
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Toxicology, 2015AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy, Phelan, Kristin M, May
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