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Essential Techniques of Cancer Cell Culture
2003A primary cell culture is the initial culture set up directly from a body tissue. Primary cancer cultures can be initiated and derived from a variety of tissue types such as solid tumor fragments (primary or metastatic) or cell suspensions, for example, aspirates, including peritoneal ascites or pleural effusions.
Kenneth G, MacLeod, Simon P, Langdon
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Techniques for Mammalian Cell Tissue Culture
Current Protocols in Human Genetics, 1994AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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General Techniques of Cell Culture
1997Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a
Maureen A. Harrison, Ian F. Rae
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Techniques for Culturing Arterial Endothelial Cells
1977Lazzarini (3) was one of the first to make use of the methods of tissue culture in the study of the nature of cells of blood vessels. His pioneer work was continued and extended by Pollack and his group (5). Like any other new approach this also had been vigorously discussed until recently.
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Optimization techniques: Studies in cell culture
Drug Development and Industrial Pharmacy, 1983AbstractWith the present large worldwide demand for vaccines and cellular by products, a number of anchorage-dependent cell mass culture systems have been developed. To obtain the maximum yield of product per run, the number of cells per system volume must be maximized and yet maintain efficiency.
E. M. Scattergood +4 more
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General Overview of Neuronal Cell Culture.
Methods in molecular biology, 2021J. Gordon, S. Amini
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Overview of Cell and Tissue Culture Techniques
Current Protocols in Pharmacology, 1999AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Cell Biology, 2007AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
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