Results 321 to 330 of about 2,687,568 (379)
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Direct microcalorimetry as a technique in cell cultures
Baillière's Clinical Endocrinology and Metabolism, 1997In vitro studies of energy metabolism in isolated cells contribute to improved knowledge of human energy metabolism under normal and pathological conditions. In every cellular system energy is taken up, metabolized and finally transformed into heat, which is dissipated into the environment.
Peter Fürst, Hartfried Böttcher
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Aseptic Technique for Cell Culture
Current Protocols in Cell Biology, 1998AbstractThis unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch‐all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality,
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Toxicology, 2015AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy Phelan, Kristin M. May
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Essential Techniques of Cancer Cell Culture
2003A primary cell culture is the initial culture set up directly from a body tissue. Primary cancer cultures can be initiated and derived from a variety of tissue types such as solid tumor fragments (primary or metastatic) or cell suspensions, for example, aspirates, including peritoneal ascites or pleural effusions.
Simon P. Langdon, Kenneth G. MacLeod
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Optimization techniques: Studies in cell culture
Drug Development and Industrial Pharmacy, 1983AbstractWith the present large worldwide demand for vaccines and cellular by products, a number of anchorage-dependent cell mass culture systems have been developed. To obtain the maximum yield of product per run, the number of cells per system volume must be maximized and yet maintain efficiency.
E. M. Scattergood +4 more
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Overview of Cell and Tissue Culture Techniques
Current Protocols in Pharmacology, 1999AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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A simple marker technique for cells in culture
Experimental Cell Research, 1964To study the interaction of two morphologically similar populations of living cells, it is necessary to distinguish them with some suitable marker. A characteristic karyotype may be used for this purpose and has the advantage that it persists during cell multiplication, but it is not possible to make direct differential counts on the living cells.
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A simplified technique for the culture of amniotic fluid cells
The Journal of Pediatrics, 1971Summary Based on studies of 40 consecutive amnioticfluid specimens, it was concluded that the most reliable and efficient technique for the culture of amniotic fluid cells is to utilize the amniotic fluid itself as the culture medium. Neither additives nor manipulation of the fluid are necessary; even gentle centrifugation and resuspension may ...
Maimon M. Cohen +2 more
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Chapter 1 Animal Cell Culture Equipment and Techniques
1998Publisher Summary This chapter describes some of the basics of cell-culture equipment and techniques, which are based on innovations, observations, realizations, accidents, mistakes, and misunderstandings encountered in cell-culture laboratories. The chapter discusses cell-culture equipment, such as hoods, incubators, microscopes, autoclaves, water ...
Angela Helmrich, David W. Barnes
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A novel cell culture technique for electron microscopy
Microscopy Research and Technique, 1993AbstractA simplified technique for the monolayer growth of cultured cells and their in situ embedment on the inner surface of the pyramidal portion of the Beem capsule for electron microscopy has been developed. The results demonstrated that the cell monolayers grew well on the surface of the Beem capsule and could be embedded in situ.
Jonathan F. Head +3 more
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