Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry [PDF]
, 2009 Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular ...
Amit Shavit +2 more
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Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation [PDF]
, 1999 Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability.
Sweeney, F. P. +6 more
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Current Approaches to Three-Dimensional Cell Culture System
Namık Kemal Tıp Dergisi, 2020 Two-dimensional (2D) cell culture is a valuable method for cell-based research, However, it may provide unpredictable, misleading data on in vivo responses.
Elif POLAT
doaj +1 more source
Modeling Central Nervous System Injury In Vitro: Current Status and Promising Future Strategies
Biomedicines, 2022 The central nervous system (CNS) injury, which occurs because of mechanical trauma or ischemia/hypoxia, is one of the main causes of mortality and morbidity in the modern society.
Kristina Pilipović +3 more
doaj +1 more source
An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]
, 2015 The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael +2 more
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Journal of Applied Oral Science, 2007 The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells.
Adriane Yaeko Togashi +4 more
doaj +1 more source
In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry [PDF]
Vojnosanitetski Pregled, 2019 Background/Aim. There is limited published evidence on the cytotoxicity of 3D printed polymer materials for dentistry applications, despite that they are now being widely used in medicine.
Puškar Tatjana +6 more
doaj +1 more source
Cytocentric measurement for regenerative medicine
Frontiers in Medical Technology, 2023 Any Regenerative Medicine (RM) business requires reliably predictable cell and tissue products. Regulatory agencies expect control and documentation. However, laboratory tissue production is currently not predictable or well-controlled. Before conditions
Alicia D. Henn +11 more
doaj +1 more source
BiomedicinesBackground/Objectives: Octenidine dihydrochloride (OCT-D) is a broad-spectrum antiseptic with high chemical stability, low toxicity, and no reported microbial resistance, making it a strong candidate for use on mucosal surfaces.
Ihsan Hakki Ciftci +4 more
doaj +1 more source
Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
, 2019 Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi +28 more
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