Results 41 to 50 of about 2,848,210 (396)

A Mathematical Model of Liver Cell Aggregation In Vitro [PDF]

open access: yes, 2008
The behavior of mammalian cells within three-dimensional structures is an area of intense biological research and underpins the efforts of tissue engineers to regenerate human tissues for clinical applications.
A. Higuchi   +57 more
core   +2 more sources

An Modified Primary Culture Method of Breast Cancer-associated Fibroblasts

open access: yesZhongliu Fangzhi Yanjiu, 2018
Objective To establish a rapid and high efficient method of primary culture of BCAFs by improving primary culture methods of common breast cancer-associated fibroblasts (BCAFs).
LI Zhiyong, BAI Xue, CAI Huiyun
doaj   +1 more source

An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]

open access: yes, 2015
The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael   +2 more
core   +1 more source

Evaluation of a composite skin substitute to treat venous stasis ulcers: a pilot study

open access: yesBrazilian Journal of Transplantation, 2006
Introduction: Venous leg ulcers represent a therapeutic challenge, and they are associated to significant morbidity and cost. Many advances have been made to develop and apply biological skin substitutes, showing they can be beneficial for patients with
Paula Dadalti-Granja   +7 more
doaj   +1 more source

Current Approaches to Three-Dimensional Cell Culture System

open access: yesNamık Kemal Tıp Dergisi, 2020
Two-dimensional (2D) cell culture is a valuable method for cell-based research, However, it may provide unpredictable, misleading data on in vivo responses.
Elif POLAT
doaj   +1 more source

Optimized Primary Culture and Subculture of Granulosa Cells

open access: yesPharmaceutical and Biomedical Research, 2022
Background: Primordial follicle includes an oocyte surrounded by a layer of somatic cells called Granulosa Cells (GCs). GCs, also known as nurse cells, are an important protective element for the growth and survival of oocytes.
Nadia Fallah   +4 more
doaj  

Development of input connections in neural cultures [PDF]

open access: yesProc Natl Acad Sci U S A. 2008 September 16; 105(37): 13758-13763, 2010
We introduce a novel approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to follow the development of the culture and see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to full term.
arxiv   +1 more source

Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates

open access: yesBioengineering, 2020
Human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells are mesenchymal-like cells, derived from human embryonic stem cells without the aid of feeder cells. They have been suggested as a potential alternative to mesenchymal stromal cells (
Sandra M. Jonsdottir-Buch   +2 more
doaj   +1 more source

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography [PDF]

open access: yesActa Biomaterialia, 51, 2017, 21-52, 2017
Unlike other tissue types, like epithelial tissue, which consist of cells with a much more homogeneous structure and function, the nervous tissue spans in a complex multilayer environment whose topographical features display a large spectrum of morphologies and size scales.
arxiv   +1 more source

Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology [PDF]

open access: yes, 2019
This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected
Araújo, Laura   +8 more
core   +2 more sources

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