Results 281 to 290 of about 12,578,619 (347)
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BIOCHEMISTRY OF CELL FRACTIONS
Canadian Journal of Biochemistry and Physiology, 1957Null
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Cell Fractionation of Parasitic Protozoa
2008Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of researchers. These studies have provided new information of the cell biology of these parasites and have supported investigators to assume that some of the protozoa form the roots of the ...
Wanderley, de Souza +2 more
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Analytical fractionation of cultured hepatoma cells (HTC cells)
Biochimica et Biophysica Acta (BBA) - General Subjects, 1978Homogenates of HTC cells have been fractionated by differential centrifugation (in four particulate fractions: N, M, L, P, and a supernatant S) or isopycnic banding in linear sucrose gradients. On this basis, the following subcellular organelles may be characterized: (i) Mitochondria, detected by cytochrome oxidase and succinodehydrogenase, are ...
P, Lopez-Saura, A, Trouet, P, Tulkens
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Biochemical Fractionation of Drosophila Cells
2007This chapter describes how to perform basic biochemical fractionations of Drosophila cells, and how to begin to characterize the proteins in the resulting fractions. The protocols include maintenance and transfection of Drosophila cell lines (Section 3.1.), hypotonic lysis (Section 3.2.), and separation of cellular lysates into cytosolic and membrane ...
Melanie, Stegman, David, Robbins
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Listeria Cell Wall Fraction: A B Cell Mitogen
The Journal of Immunology, 1975Abstract A crude cell wall-rich fraction of Listeria monocytogenes contains a substance or substances which cause blast transformation and thymidine incorporation by mouse spleen cells. The substance, Listeria cell wall fraction (LCWF), is a B cell mitogen in the mouse.
Cohen, J J +3 more
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Specific Fractionation of Immunocompetent Cells
Immunological Reviews, 1970Prominent progress in molecular biology was only made when it became possible to separate functionally distinct molecules by taking advantage of their biophysical properties. Likewise, the analysis of the functions of heter ogeneous populations of immunocompetent cells, as to the functional properties of their various subpopulations, can not be done ...
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Lipolytic activity in adipocyte cell fractions
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Adipocytes release only negligible amounts of free fatty acids unless stimulated, but reveal considerable lipolytic activity when homogenized. Epinephrine treatment of the cells caused only a 20-40% increase in the activity of infranatants of homogenates while raising the activity associated with the fat layer up to 10-fold. Full activity (i.e. that of
Y, Oschry, B, Shapiro
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Subcellular fractionation of tissue culture cells
Trends in Biochemical Sciences, 1989Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the ...
K E, Howell, E, Devaney, J, Gruenberg
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Differential Detergent Fractionation of Eukaryotic Cells
Cold Spring Harbor Protocols, 2005INTRODUCTIONDifferential detergent fractionation (DDF) involves the sequential extraction of cells with PIPES buffers containing first digitonin, then Triton, and finally Tween/deoxycholate (DOC). The procedure yields four biochemically and electrophoretically distinct fractions composed of the following: (1) cytosolic proteins and extractable ...
Melinda, Ramsby, Gregory, Makowski
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Cell cycle analysis by culture fractionation
Experimental Cell Research, 1972Abstract The isolation of age-related cell size classes from cultures of the yeast, Schizosaccharomyces pombe, was carried out in a reorienting gradient zonal rotor. Measurements on cell growth, septa formation, and cell division from time-lapse studies were used to establish the average ages of fractions following culture fractionation.
J R, Wells, T W, James
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