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Lipolytic activity in adipocyte cell fractions
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Adipocytes release only negligible amounts of free fatty acids unless stimulated, but reveal considerable lipolytic activity when homogenized. Epinephrine treatment of the cells caused only a 20-40% increase in the activity of infranatants of homogenates while raising the activity associated with the fat layer up to 10-fold. Full activity (i.e. that of
Y, Oschry, B, Shapiro
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Cell fractionation of large bowel cancer
Cancer, 1977The fractionation of cells from human colonic carcinoma, a transplantable colonic carcinoma of the rat, and normal rat colonic mumsa by centrifugation in linear gradients of Ficoll (polysucrose) in tissue culture medium is described. Epithelial cells from human colonic carcinoma showed strong histochemical N-acetyl-@-D-glucosaminidase activity relative
M G, Brattain +2 more
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Subcellular fractionation of tissue culture cells
Trends in Biochemical Sciences, 1989Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the ...
K E, Howell, E, Devaney, J, Gruenberg
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Differential Detergent Fractionation of Eukaryotic Cells
Cold Spring Harbor Protocols, 2005INTRODUCTIONDifferential detergent fractionation (DDF) involves the sequential extraction of cells with PIPES buffers containing first digitonin, then Triton, and finally Tween/deoxycholate (DOC). The procedure yields four biochemically and electrophoretically distinct fractions composed of the following: (1) cytosolic proteins and extractable ...
Melinda, Ramsby, Gregory, Makowski
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Cell cycle analysis by culture fractionation
Experimental Cell Research, 1972Abstract The isolation of age-related cell size classes from cultures of the yeast, Schizosaccharomyces pombe, was carried out in a reorienting gradient zonal rotor. Measurements on cell growth, septa formation, and cell division from time-lapse studies were used to establish the average ages of fractions following culture fractionation.
J R, Wells, T W, James
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Catecholamine release from fractionated chromaffin cells
European Journal of Pharmacology, 1996Bovine chromaffin cells were separated by density gradient centrifugation into subfractions. After centrifugation on a self-generating Percoll gradient (42.75% isotonic Percoll, 30,000 x g for 22 min at 20 degrees C), the chromaffin cells were found in two clearly distinguishable peaks.
W, Krause +4 more
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Cell Disruption and Fractionation
2011The living cell contains a number of subcellular fractions. The fractionation of cells involves two distinct phases: disruption of the tissue or cells in a suitable medium and the subsequent separation of the subcellular particles, by differential centrifugation which exploits differences in their size and density. The procedure results in rather crude
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Electron Microscopy: Cytology of Cell Fractions
Science, 1956It should be evident from this brief account that electron microscopy of thin sections is an invaluable asset in the study of fractions isolated by differential centrifugation. I have tried to indicate how the integrity of particles, purity of fractions, and the existence of new particles can be established through its use.
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