Results 1 to 10 of about 90,745 (166)

LAFITE Reveals the Complexity of Transcript Isoforms in Subcellular Fractions [PDF]

open access: yesAdvanced Science, 2023
Characterization of the subcellular distribution of RNA is essential for understanding the molecular basis of biological processes. Here, the subcellular nanopore direct RNA‐sequencing (DRS) of four lung cancer cell lines (A549, H1975, H358, and HCC4006)
Jizhou Zhang   +7 more
doaj   +2 more sources

Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis [PDF]

open access: yesBio-Protocol, 2018
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity.
Sébastien Gillotin
doaj   +2 more sources

Veterinary fluoroquinolones as emerging contaminants in marine environments: In vitro study of biochemical responses in subcellular fractions of the Mediterranean mussel (Mytilus galloprovincialis) [PDF]

open access: yesHeliyon
Fluoroquinolone antibiotics (FQs) are emerging pollutants frequently detected in aquatic environments. However, their impact on marine invertebrates remains underexplored.
J. Giannessi   +6 more
doaj   +2 more sources

Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions

open access: yesBio-Protocol, 2021
Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that
Ankita Arora   +3 more
doaj   +1 more source

Application of RNA subcellular fraction estimation method to explore RNA localization regulation

open access: yesG3: Genes, Genomes, Genetics, 2021
RNA localization is involved in multiple biological processes. Recent advances in subcellular fractionation-based sequencing approaches uncovered localization pattern on a global scale. Most of existing methods adopt relative localization ratios (such as
Xiaomin Dai   +8 more
doaj   +1 more source

Development of a UPLC-MRM-based targeted proteomic method to profile subcellular organelle marker proteins from human liver tissues

open access: yesScientific Reports, 2022
Subcellular organelles have long been an interest in biochemical research and drug development as the isolation of those organelles can help to probe protein functions and elucidate drug disposition within the cell.
Xiazi Qiu   +2 more
doaj   +1 more source

Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer’s Disease

open access: yesBiomedicines, 2021
Alzheimer’s disease (AD) is characterized by the accumulation of extracellular plaques composed by amyloid-β (Aβ) and intracellular neurofibrillary tangles of hyperphosphorylated tau.
Tânia Fernandes   +8 more
doaj   +1 more source

Subcellular partitioning of protein kinase activity revealed by functional kinome profiling

open access: yesScientific Reports, 2022
Protein kinases and their substrates form signaling networks partitioned across subcellular compartments to facilitate critical biological processes. While the subcellular roles of many individual kinases have been elucidated, a comprehensive assessment ...
Lauren Wegman-Points   +6 more
doaj   +1 more source

Distribution of polycyclic aromatic hydrocarbons in subcellular root tissues of ryegrass (Lolium multiflorum Lam.)

open access: yesBMC Plant Biology, 2010
Background Because of the increasing quantity and high toxicity to humans of polycyclic aromatic hydrocarbons (PAHs) in the environment, several bioremediation mechanisms and protocols have been investigated to restore PAH-contaminated sites.
Zhang Yi   +3 more
doaj   +1 more source

The subcellular distribution of platelet lipids labeled by acetate-l-14C

open access: yesJournal of Lipid Research, 1971
The lipids of intact human platelets were labeled in vitro with acetate-1-14C, and the distribution of radioactivity in individual fatty acids and in lipid classes was examined in platelet subcellular fractions separated by sucrose density gradient ...
DANIEL DEYKIN
doaj   +1 more source

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