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Immuno-serological diagnostic tools particularly identifying pathogen antigens are the most important methods of pseudotuberculosis studies. The main immunodominant and species-specific antigens located in the surface structures of the bacterial cell are
Anna V. Kryukova +9 more
doaj +1 more source
Subcellular Fractionation and Proteomics of Nuclear Envelopes [PDF]
Because of its many connections to other cell systems, the nuclear envelope (NE)is essentially impossible to purify to homogeneity. To circumvent these problems, we developed a subtractive proteomics approach in which the fraction of interest and a fraction known to contaminate the fraction of interest are separately analyzed, and proteins identified ...
Florens, Laurence +2 more
openaire +3 more sources
Binding of radioactive methylmercuric chloride in subcellular fractions of neuroblastoma and glioma cells in culture [PDF]
The binding of radioactive methylmercuric chloride ([203Hg] -CH3HgCl) with various subcellular fractions of glioma (C-6) and neuroblastoma (NBP2) cells was studied. Confluent cells were incubated in the presence of radioactive CH3HgCl for a period of 5 h,
Prasad, KN, Ramanujam, M, Bondy, SC
core +1 more source
HMG CoA reductase of intestinal mucosa and liver of the rat
Methods were developed for the determination of HMG CoA (3-hydroxy-3-methylglutaryl CoA) reductase activity in subcellular fractions of intestinal mucosa and liver of Wistar strain rats.
S. Shefer +3 more
doaj +1 more source
Low density subcellular fractions enhance disease-specific prion protein misfolding
The production of prion particles in vitro by amplification with or without exogenous seed typically results in infectivity titers less than those associated with PrPSc isolated ex vivo and highlights the potential role of co-factors that can catalyze ...
James F. Graham (17218012) +5 more
core +3 more sources
Lipid composition of subcellular particles of human blood platelets
Human platelets can be fractionated into three main subcellular components: granules, membranes, and a soluble fraction. In this study we determined the phospholipid and neutral lipid content of the granules and membranes.
Aaron J. Marcus +2 more
doaj +1 more source
De novo synthesis and elongation of fatty acids by subcellular fractions of monkey aorta
Subcellular fractions of aorta of squirrel monkey (Saimiri sciureus) were examined for their ability to synthesize and elongate fatty acids. High-speed supernate (HSS) incorporated substantial quantities of malonyl CoA into fatty acids while acetyl CoA ...
Charles F. Howard, Jr.
doaj +1 more source
Characterization of subcellular fractions in control and MβCD treated myocytes.
(1A) A representative blot demonstrating the subcellular distribution of α-actinin and cytochrome c oxidase subunit IV (cyt c ox) in time control myocytes (lanes 1 and 2) and myocytes treated with MβCD (lanes 3 and 4). (1B) Western blot demonstrating the
Mohammed Z. Haque (3148443) +4 more
core +1 more source
During myocardial ischemia increased levels of lysoglycerophospholipids have been reported which may be deleterious to myocardial function. Phospholipases are presumed to be important in the regulation of this process.
G Nalbone, K Y Hostetler
doaj +1 more source
Immunochemical or fluorescent labeling of vesicular subcellular fractions for microscopy imaging
We describe a procedure for the labeling of membranous vesicular purified subcellular fractions, to image them, typically by confocal laser scanning microscopy.
RAVERA, SILVIA +4 more
core +1 more source

